Precise Regulation Of Nucleic Acid-mediated Membrane Protein Aggregation

The structure of a single protein molecule determines its physicochemical properties,and the aggregation of multiple proteins is the basis for its realization of many biological functions.When this aggregation reaches a certain level,phase separation will occur.At present,a number of studies have been involved,but the research is limited to the observation and analysis of protein phase separation.The control methods for the agglomeration behavior of proteins are still scarce.Herein,a precise regulation method for nucleic acid-mediated molecular spatial arrangement was established,and the effective manipulation of cell surface protein molecular aggregation behavior was realized by this method.We have achieved precise gold nanoparticle spatial arrangement by introducing a triangular DNA origami as a substrate.We used the base complementation of nucleic acids to achieve multiple modifications on the cell surface and established a novel nucleic acid-encoded CAR-T cell.We use the tetrahedral nucleic acid structure as a motif to control the aggregation behavior of antibody molecules by manipulating the self-assembly between motifs.This strategy provides a new direction for the manipulation of protein aggregation behavior,and is expected to become an important research tool in the field of phase separation in the future..