Experimental Method And Technology Research Of Orthogonal Phase Imaging

As the basic and functional unit of organism,cells have a variety of complex forms,especially in the different individuals and health states,the difference between individuals is particularly significant.With the further research of scientists in modern medicine,it is found that cell morphological information plays a crucial role in disease detection,diagnosis,treatment and new drug development.Therefore,cell imaging technology becomes an effective way to collect cell morphology.Optical phase imaging technology is widely applied because of its ability to detect low scattering phase samples,like cells.In particular,quantitative phase microscopy has made significant progress of the past ten years.The technology,which integrates digital holography,optical microscopy and modern digital processing technology,can achieve non-invasive,non-radiative,non-contact,hitless and real-time quantitative measurement,through analyzing the transmitted or reflected light of the sample.It has become one of the main technologies in the field of cell detection because of its good nanometer measurement accuracy.Through reading the related data of a large number of phase microscopy and phase recovery methods,the shortcomings of the traditional imaging techniques are summarized and compared.On the basis of off-axis and coaxial interferometric phase imaging technique,a coaxial and off-axis interferometric imaging system based on the dual Dove prism based on Michelson interference optical path is proposed in this paper to give full play to the characteristics of the previously methods.It can carry out coaxial and off-axis interference phase imaging,and at least employs lenses,which effectively reduce optical distortion.The wide practicability and popularization significance has been showed because of the simple optical structure,convenient operation and low cost of the method in phase microscopy.But in traditional phase microscopic imaging technology,the pseudo thickness distribution of samples in axial direction can only be obtained through reconstruction.In order to get a better,clearer and more realistic three-dimensional substructure information of biological cell samples,we need to collect and analyze more phase maps for reconstruction.In this paper,in order to obtain the complete three-dimensional information of the sample cells based on the maximum entropy method,an orthogonal interference imaging system based on biorthogonal optical path is proposed by using the lateral displacement prism.It can obtain two interference patterns in the two orthogonal direction at the same time,and can obtain the three-dimensional morphology of the biological cells and its substructure characteristics by the correlation phase recovery method.Different from the traditional off-axis interferometric imaging technology,the phase information of the sample can only be collected and stacked in axial direction.This method only needs to recover all the spatial information of the sample by the two interference patterns collected from the orthogonal direction.Its authenticity and reliability are verified by computer simulation and experimental results.This technique can be widely used in phase microscopy,especially in phase microscopy of transparent samples,such as phase imaging of biologic cells and phase measurement.Two different imaging systems are put forward in this paper will enable us to successfully perform clear imaging and explore new ideas for further research on threedimensional orthogonal imaging of biological cell samples.