| OBJECTIVE: Biomimetic micropatterned PDMS surfaces of clover,bamboo leaf,creepers and fingerprint were constructed on the surface of PDMS,and the surface morphology,roughness and water wetting angle of four biomimetic micropatterned PDMS surfaces were characterized.The effects of different biomimetic micropatterned PDMS surfaces on the morphology,proliferation and adhesion of Cal-27 and Hela cells were compared through related cell experiments,so as to prevent malignant tumors.Diagnosis and treatment provide a new research perspective.Methods: Using clover,bamboo leaf,creepers and fingerprint as templates,biomimetic micro-patterned PDMS of clover,bamboo leaf,creepers and fingerprint was constructed on the surface of PDMS.The surface morphologies of four biomimetic micropatterned PDMS and four original templates were characterized by SEM;the surface morphologies of four biomimetic micropatterned PDMS were characterized by three-dimensional profilometer and their roughness was measured;the water contact angles of blank PDMS and four biomimetic micropatterned PDMS surfaces were measured by contact angle measuring instrument.The toxicity of PDMS materials to Cal-27 and Hela cells was evaluated by MTT assay.The morphological effects of four bionic micropatterned PDMS surfaces on Cal-27 and Hela cells were observed by SEM,and the effects of four bionic micropatterned PDMS surfaces on the morphology and growth trend of Cal-27 and Hela cells were evaluated by fluorescence microscopy.RESULTS:(1)Physical characterization: SEM observation showed that the bionic micro-patterns on PDMS surface were similar to those of the original template epidermis and presented complementary morphological structure.The surface patterns of Parthenocissus Tigris were many irregular long "island" structural units.The surface morphology of Clover was highly symmetrical,similar to that of many "elliptical plates" arranged densely in a "fish scale" shape,and the surface patterns of bamboo leaves were in a "fish scale" shape.The results of surface roughness measurement by three-dimensional profilometer showed that the surface roughness of micropatterned PDMS of Parthenocissus crassipes was 2.44(+0.32)micropatterned;the surface roughness of bamboo leaf micropatterned PDMS was 5.53(+0.57)micropatterned PDMS;the surface roughness of clover micropatterned PDMS was7.12(+0.48)micropatterned PDMS;and the surface roughness of fingerprint micropatterned PDMS was 7.12(+0.48)micropatterned.The surface roughness is10.50 +0.72 um.The surface roughness of four biomimetic micropatterned PDMS is as follows: the surface roughness of mountain tiger micropatterned PDMS < bamboo leaf micropatterned PDMS < clover micropatterned PDMS < fingerprint micropatterned PDMS surface;the contact angle of four biomimetic micropatterned PDMS surface measured by contact angle measuring instrument shows that the water contact angle of blank PDMS surface is about 108.32(+0.08);.On the surface of tiger micropatterned PDMS,the water contact angle is about 110.64(+0.16 degrees);on the surface of fingerprint micropatterned PDMS,the water contact angle is about113.56(+0.10 degrees);on the surface of bamboo leaf micropatterned PDMS,the water contact angle is about 114.93(+0.22 degrees);and on the surface of clover micropatterned PDMS,the water contact angle is about 116.75(+0.18 degrees).PDMS is a kind of hydrophobic material.Its surface is a hydrophobic interface.The hydrophobicity of PDMS surface is enhanced by the construction of biomimetic micropatterned PDMS surface.In a certain range,with the increase of roughness,the hydrophobicity of micropatterned PDMS surface tends to increase.(2)Cell experiment: MTT results showed that the survival rates of the four biomimetic micropatterned PDMS surfaces decreased with the increase of surface roughness.Cal-27 cells were more sensitive to the surface roughness of biomimetic micropatterned PDMS than Hela cells.The results of SEM and fluorescence microscopy showed that biomimetic micropatterned PDMS affected the morphology and characteristics of Cal-27 and Hela cells.The surface of biomimetic micropatterned PDMS could promote the spreading and adhesion of Cal-27 and Hela cells.For the fingerprint micropatterned PDMS and bamboo leaf micropatterned PDMS with obvious orientation,most of the two cells spread along the orientation of the pattern.Within a certain range of roughness,the smaller the surface roughness of biomimetic micropatterned PDMS is,the smaller the nuclear-cytoplasmic ratio ofCal-27 cells and Hela cells is,the more conducive to cell spreading.CONCLUSION: Four kinds of biomimetic micropatterned PDMS surfaces were successfully constructed on the surface of PDMS.The surface structure can promote the adhesion and spreading of Cal-27 and Hela cells.Within a certain range of roughness,the smaller the surface roughness of biomimetic micropatterned PDMS,the smaller the nuclear-cytoplasmic ratio of Cal-27 cells and Hela cells,the more favorable the spreading of Cal-27 cells and Hela cells.For patterns with obvious orientation,more cells are found.Spread along the orientation of the pattern.Cal-27 cells are more sensitive to the surface morphology of biomimetic micropatterned PDMS than Hela cells. |
