Seasonal Dynamics Of Soil Fungi And Enzymatic Activity In Spruce (Picea Crassifolia) Forests Of Qilian Mountains

Picea crassifolia forest is the main type of water conservation forest in Qilian mountains,and is the main part of Hexi corridor oasis and silk road culture.So far,little research has been done on the seasonal dynamics of soil fungi community and soil enzyme activities in spruce forest of Qinghai.In this study,soil samples were collected in different seasons from spruce forest in Xidatan township,Tianzhu county,Gansu province.By using high-throughput sequencing technology and combining with soil physical and chemical properties and meteorological conditions,seasonal dynamic changes of fungi,different functional groups and enzyme activities at different classification levels in soil were studied.The main findings are as follows:There were significant seasonal differences in soil fungal communities.The richness and diversity of fungal communities were significantly higher in summer than in winter,and the relative abundance of Ascomycota and Zygomycota was higher in summer than in winter.The relative abundance of Basidiomycetes was lower in summer than in winter.The correlation with environmental factors indicated that soil ammonium nitrogen,air temperature and precipitation in different seasons were significantly correlated with soil fungal community structure.The relative abundance of symbiotic fungi in summer was lower than that in winter.On the contrary,the relative abundance of pathogenic fungi and saprophytic fungi in summer was higher than that in winter.There was a significant correlation between the seasonal changes of soil fungal functional groups and environmental factors such as ammonium nitrogen,air temperature and precipitation.There was also a significant correlation between the functional groups of fungi.Soil enzyme activities showed strong seasonal differences,in which ?-glucosidase was closely related to the environment and fungal community,and had a strong positive correlation with soil water content,organic carbon and total nitrogen,and a significant negative correlation with the relative abundance of Ascomycetes.