Construction And Analysis The Networks Of LncRNA As CeRNA During Mouse Spermatogenesis

A variety of male reproductive diseases are closely related to the process of spermatogenesis.There is great research value to explore the molecular regulation mechanism of spermatogenesis.Long noncoding RNA(lncRNA)is a kind of non-coding RNA with nucleotides length more than 200,which has spatiotemporal specific expression in different growth and development processes as well as tissues and cell types.A study shown that some lncRNAs are specifically expressed in testis tissue,suggesting that they may have potential regulatory functions during spermatogenesis.However,only a small part of lncRNA has functional or mechanistic studies,and there is no systematic analysis of the function of lncRNAs in spermatogenesis.In recent years,studies shown that lncRNA can be involved in the regulation of gene expression as a competitive endogenous RNA(ceRNA).By binding to miRNAs,ceRNA acts as a molecular "sponge" for miRNAs,relieving the inhibition and degradation of miRNAs on their target genes.It plays an important regulatory role in some biological phenomena such as cell development,differentiation and disease occurrence.However,it is unclear whether lncRNA specifically expressed during spermatogenesis will play a regulatory role in ceRNA.Therefore,based on the RNA-seq data in mouse spermatogenesis,we constructed a regulatory network of lncRNA-associated ceRNAs during mouse spermatogenesis and explored the relationship between ceRNA networks and transcriptional regulation of spermatogenesis.1.Analysis of dynamic expression of genes during mouse spermatogenesis based on RNA-seq dataBased on the public data obtained from the GEO database,by analyzing the dynamic expression changes during spermatogenesis,we obtained 3478 and 851 mRNAs and lncRNAs that differentially expressed in spermatogonia and spermatocytes,941 and 489 mRNAs and lncRNAs differentially expressed in spermatocytes and round sperm.Functional enrichment analysis revealed that phase-specific mRNAs were significantly enriched in the pathways involved in spermatogenesis.2.Construction of ceRNA network related to mouse spermatogenesisIntegrating the miRNAs-genes regulatory relationship provided by databases such as miRDB and DIANA-LncBase,combined with the expression data,constructed a lncRNA-related ceRNA regulatory network during spermatogenesis.In the end,the study explored 1343 specific ceRNA relationship pairs during spermatogenesis,including 324 lncRNAs and 430 mRNAs.Functional enrichment analysis revealed that mRNAs enrichment in this ceRNA network are involved in spermatogenesis-related pathways.3.Analysis of ceRNA network in mouse spermatogenesisIn this study,clustering analysis of mRNAs in ceRNA networks revealed that different clusters exhibited different expression patterns and associated with different biological functions.Combined with relevant literature and databases,some of the genes in networks have been confirmed to be transcription factors associated with spermatogenesis.This suggests that the ceRNA network and the transcription factor regulatory network are closely related during spermatogenesis,they work synergistically.Finally,the study screened some of the more possible and more sensitive pairs of ceRNAs for downstream experiments.This study explores the potential regulatory role of lncRNA in spermatogenesis by constructing a regulatory network of ceRNA related to lncRNAs during the spermatogenesis,and expands the understanding of the regulation methods of lncRNAs,providing a preliminary theoretical basis for further exploring the molecular regulation mechanism of spermatogenesis.