Study On Bacterial Culture Chip And Sensitivity Analysis Of Antibiotics Based On Battlement Structure Devices

The high production and unreasonable use of antibiotics has made environmental pollution and drug resistance problems increasingly serious.Conventional bacterial susceptibility testing methods are time consuming,labor intensive,and low in sensitivity.The use of automated antibiotic susceptibility testing instruments for bacterial susceptibility testing is not only expensive but also costly to maintain and does not allow real-time monitoring of bacterial growth.Compared with the traditional bacterial sensitivity test method,the microfluidic technology can not only accurately control the culture conditions,but also observe the growth state of the bacteria in real time,and can greatly reduce the culture cost and time,and shorten the measurement cycle of the minimum inhibitory concentration of antibiotics.In this paper,a microfluidic research platform adapted to the research environment microorganisms was established,and a microfluidic chip with dynamic and stable microenvironment was designed to realize rapid culture of bacteria,real-time observation of bacteria in situ and antibiotic sensitivity analysis.The minimum inhibitory concentration.More morphological information is obtained than conventional methods.Firstly,the microfluidic research platform was constructed,including the power system,chip system and observation system.Five kinds of continuous flow bacterial culture chips were designed to cultivate the micro-outdoor set gap,which is similar to the battlement.The COMSOL software was used to simulate the flow velocity distribution in different culture chambers.The ability of the five structures to resist external liquid perturbation was analyzed.The structure with small flow velocity and uniform distribution in the culture chamber was selected.It was found that there was a shunt structure at the periphery and it was internally The dynamic changes in the culture chamber of the battlement are relatively stable.Three kinds of microfluidic chips were prepared,and the bacteria observation and growth curve drawing method of the microfluidic chip were established.On the basis of COMSOL simulation,the research on rapid culture of bacteria based on chip was carried out,and the optimal conditions suitable for bacterial culture were explored.Studies have shown that the optimal structure for culture of Escherichia coli and Pseudomonas aeruginosa by microfluidic chip is structure IV,the optimum flow rate is 0.1 ?L/min,0.5 ?L/min,and the optimum medium is LB medium.Compared with the conventional well plate method,the microfluidic chip-based bacterial culture is superior to the conventional well plate method,and has the advantages of realizing rapid culture of bacteria,reducing reagent consumption,and observing bacterial growth state in real time.The microfluidic chip was used to study the inhibition process of antibiotics on bacteria.Firstly,the inhibition curves of three antibiotics on two kinds of bacteria were obtained by using the well plate experiment.Then,the micro-fluidic chip was used to quickly obtain the inhibition characteristics and morphological changes of antibiotics against Escherichia coli and Pseudomonas aeruginosa.The minimum inhibitory concentration of Escherichia coli under tetracycline hydrochloride and erythromycin was 12mg/L and 100mg/L.The minimum inhibitory concentration of Pseudomonas aeruginosa under tetracycline hydrochloride,erythromycin and ciprofloxacin was 30mg/L,300 mg/L,0.7 mg/L.This result is similar to the results obtained by the conventional well plate method.It proves that the microfluidic chip is a reliable means to quickly determine the minimum inhibitory concentration of bacteria,shorten the test time,and is easy to operate.It can be applied to study the effect of new environmental pollutants to bacterial growth.