Regulatory Function Of Tartary Buckwheat Protein And Lactobacillus Plantarum On Blood Lipid And Intestinal Flora In Mice

Tartary buckwheat protein was well known as its low digestibility,which has been found to have a variety of beneficial effects,such as reducing blood lipid,resisting oxidation and regulating intestinal flora.Lactobacillus plantarum ST-? not only has the effect of improving the flavor of yogurt,but also has the effect of reducing cholesterol and inhibiting the growth of intestinal pathogenic bacteria.At present,there are few reports on the study of tartary buckwheat protein and Lactobacillus plantarum ST-?,especially the synergistic regulation of lipid metabolism and intestinal flora in high-fat diet mice.In this paper,the dietary intervention of high-fat diet mice was carried out by using tartary buckwheat protein protein,Lactobacillus plantarum ST-?,and a combination of the two.Male C57BL/6 mice were divided into low fat diet group(LFD),high fat diet group(HFD),Lactobacillus plantarum ST-? intervention group(HFD+LP),Tartary buckwheat protein intervention group(BWP),Tartary buckwheat protein and Lactobacillus plantarum ST-? synergistic intervention group(BWP+LP).Animals were all fed with high-fat diet,except for the LFD group.And the dietary intervention was conducted for 6 weeks.Changes of blood lipid metabolism,organ oxidative stress and intestinal flora in experimental animals were analyzed and compared,through which we can find the best intervention mode for the intervention of the disorder of blood lipid metabolism and intestinal flora in high-fat diet mice.The main results are as follows:(1)Blood lipid index,antioxidant index,total lipase and transaminase activity,inflammatory factors and other indexes of mice were determined,so as to analyze the regulatory effects of the three intervention methods on the disorder of blood lipid metabolism in high-fat diet mice.Results showed that the three means of intervention could significantly reduce the blood lipid level of mice a high-fat diet(TC,LDL-C,AI),improve antioxidant capacity(GSH,MDA),enhance liver function related enzyme(LPL,HL,ALT,AST)enzyme activity,and decrease the plasma inflammatory cytokines(LPS,IL-6,TNF-?)level(P<0.05).Therefore,all three intervention methods can regulate the disorder of blood lipid in mice caused by high-fat diet.Meanwhile,comparative analysis showed that the synergistic intervention effect of Tartary buckwheat protein and Lactobacillus plantarum ST-? was superior to the individual intervention effect of the two.(2)Sections of mice tissues(liver,colon and duodenum)were analyzed,and antioxidant indexes of tissues and inflammatory factors of colon were measured in this part.And the regulatory effects of three intervention methods on the occurrence of organ injury and inflammation in mice were explored.Results showed that the high-fat diet could cause the inflammation of the mice organs(liver,colon,duodenum),destroy antioxidant capacity of mice and increase the level of colonic inflammatory factors in mice.Compared with the two individual intervention methods,these pathological changes in mice caused by high-fat diet could be more effectively and comprehensively inhibited by the synergistic intervention of Tartary buckwheat protein and Lactobacillus plantarum ST-?.So that the mice body could be more close to the mice fed with low-fat and low-sugar diet.(3)Changes in intestinal flora and contents of short-chain fatty acids(SCFAs)in mice feces were analyzed by high-throughput sequencing and GC-MS technology,so as to evaluate the regulatory effects of three intervention methods on intestinal flora disorders of mice with high-fat diet.Results showed that the high-fat diet caused intestinal flora disorder in mice,and the composition of the flora was changed,the Firmicutes/Bacteroidetes(F/B)value was increased,and the SCFAs content in mouse feces were significantly decreased(P<0.05).Combined with species composition analysis and sample comparison analysis,the intestinal microflora of mice in the BWP+LP group was more similar to that in the LFD group,including that intestinal microflora disorder of mice caused by high-fat diet could be more effectively regulate by the synergistic intervention of Tartary buckwheat protein and Lactobacillus plantarum ST-?.Therefore,the composition of intestinal flora could be more similar to the normal composition of low-fat diet mice.Meanwhile,the synergistic intervention could promote the growth of SCFAs to optimize the intestinal environment and protect the intestinal health.In summary,the disorder of blood lipid metabolism,liver and intestinal damage and the change of intestinal flora balance in mice caused by high-fat diet could be effectively regulated by the synergistic intervention of tartary buckwheat protein and Lactobacillus plantarum ST-?.Besides,the regulatory effect of the coordinated intervention is better than that of the two alone.