Green Synthesis Of Silver Nanoparticles By Using Bacterial Extract And Its Antibacterial Activity

Nanotechnology is the amendment,alteration,and development of significant properties of metals in the form of nanoparticles having applications in numerous fields.Nanoparticles are at the leading position of the fast progress of nanotechnology.Adaptation of eco-friendly and reliable technique for the biosynthesis of nanoparticles is a field of nanotechnology.Biosynthesis of nanoparticles is an eco-friendly and important field of nanotechnology.The aim of this study is to isolate bacteria from non-polluted forest environment and use bacterial extracts to synthesize silver nanoparticles.In this study,10 strains of bacteria were isolated from Ninglin District of Zhaobi Shanxi Province.It was found that T10 could synthesize silver nanoparticles,and was identified as Shigella flexneri by physiological,biochemical and molecular identification.AgNPs were synthesized from T10 extract and characterized by scanning electron microscopy,X-ray diffraction,infrared spectroscopy and chemical characterization of its size,shape and structure.The results were as follows: The T10 strain can synthesis AgPs within one hour of incubation,stable and dispersed AgNPs were obtained in vitro with bacterial extracts.The reduction of silver ion was analyzed by using UV–visible spectrophotometry,and characterization of the AgNPs were done by Scanning electron microscopy,X-ray diffraction and Fourier transform infrared spectroscopy.These nanoparticles were monodispersed,spherical and about 36–42.7 nm in size and properly caped by the bacterial extract.The X-ray diffraction peaks were obtained at 2? angels of 38.6°,44°,64.44°,and 77.16° were corresponding to diffraction facets of silver planes.The capping of AgNPs with functional groups were confirmed by FTIR.The obtained AgNPs showed antimicrobial activity against clinical pathogens Candida albican,Staphylococcus aureus,Klebsiella pneumonia,Klebber spp,E.coil,and Staphylococcus aureus(MRSA)and synergistic effect with antibiotics.Antibacterial activity was significantly improved by applying AgNPs on the bandages.In conclusion,the Shigella flexneri from unusual environment have the capability of synthesizing AGNPs.Hence,our results provide that bacterial strains from extreme environment have high AgNPs producing capacity.