Faraday Measurement Of Serum And DNA Damage Under Electromagnetic Radiation

In recent years,with the development of radio communication technology and the popularization of electronic products and equipment,electromagnetic radiation has gradually become a hidden danger affecting human health.With the increasing intensity of electromagnetic radiation in the environment year by year,electromagnetic biological effects have become the focus of attention of scholars at home and abroad,and have been extensively studied in many interdisciplinary fields such as electromagnetic biology.Among them,the damage of biological macromolecules such as DNA induced by electromagnetic radiation is the core of the study of electromagnetic biological effects.DNA and protein are the basic life substances of human body.DNA carries all the genetic information of the whole organism.It guides the synthesis of proteins needed for life activities through the process of replication and transcription,and maintains the basic life activities and growth and development.Therefore,the integrity of DNA molecular structure is of great significance to cells and even the whole organism.When the human body is exposed to electromagnetic radiation for a long time,the free-moving charges in the cells of the body will be affected by both external electric field force and Lorentz force.When the superimposed force is greater than the intermolecular force that maintains the stability of the microstructures of DNA and protein,it may cause structural damage to varying degrees.Under the action of electromagnetic dipole electronization,the body will be damaged to varying degrees.The non-linear resonance effect in vivo is easily caused by the constant friction and collision between the various groups of molecules.Moreover,the biological effects of electromagnetic radiation on human body are related to the frequency and intensity of electromagnetic waves.The different biological effects of electromagnetic radiation mean different mechanisms of action.DNA damage may lead to apoptosis,gene mutation and so on,which can induce organisms with different symptoms.The effects may eventually induce various diseases.Therefore,the study of electromagnetic radiation on DNA has great reference significance and practical value for the application of electromagnetic wave in biomedicine.Taking calf thymus DNA and bovine serum albumin molecule(BSA)as research objects,the mechanism of action of calf thymus DNA and bovine serum albumin molecule(BSA)under differenttemperature,different laser wavelength and different magnetic field intensity was studied,and a Faraday method for measuring the evolution process of radiation physical properties of calf thymus DNA and bovine serum albumin solution in the field was proposed.The main research contents are as follows:(1)The physical and chemical properties of DNA and serum are introduced,and the theoretical basis of Faraday optical rotation effect measurement is also introduced;(2)The Faraday optical rotation effect measurement system is constructed,the experimental measurement scheme is designed,the sources of experimental errors and the compensation measures to reduce experimental errors are analyzed;(3)The optical rotation characteristics of DNA and the variation laws of different light wavelength,magnetic field intensity and temperature are obtained.On this basis,the specific optical rotation and Fidel constant of DNA are calculated.The Faraday anomalous dispersion characteristics of DNA are obtained,and the effects of electromagnetic field on the physical properties of DNA are studied and analyzed;(4)The natural optical rotation and Faraday optical rotation characteristics of bovine serum albumin(BSA)were studied.The effects of wavelength,concentration and temperature on the natural optical rotation characteristics of BSA were analyzed.The Faraday magnetic rotation characteristics of BSA under different magnetic field intensities were measured.The specific optical rotation and Fidel constant of BSA were obtained.The effects of electromagnetic field on the physical properties of BSA were discussed.The purpose of this paper is to propose a Faraday optical rotation method for measuring the effects of electromagnetic field radiation on the physical properties of DNA and serum albumin,and to reveal the optical rotation characteristics of DNA and serum albumin under different magnetic fields at different temperatures,wavelengths and intensities,including the variation of specific optical rotation and Fedel constant.Compared with Giemsa staining method,TUNUL method,DNA electrophoresis gradient method and flow cytometry method,Faraday polarimetry method has the advantages of convenience,rapidity,high accuracy and real-time measurement.