Response Mechanism Of Soil Microorganisms To Livestock Grazing In Alpine Grassland

Soil microorganisms are the most important and active component of the soil ecosystem.The relationship between soil microbial community composition,diversity change and ecosystem function and its stability is a hot research topic in the field of life science nowadays.Soil microorganisms play an important role in the functional maintenance and sustainable development of ecosystems,and the community composition and diversity of soil microorganisms can reflect the changes of soil ecosystem to some extent.In this paper,the composition and diversity of soil microbial communities in alpine grassland ecosystem under different management measures were systematically studied by using high-throughput sequencing technology.The changes of soil microbial community composition and diversity in alpine grassland(MY stands Haibei stantion,WY stands WaYan mountain stantion,YC stands ZhongYangchang station)under different management measures(fencing and grazing)were analyzed.The response of soil microorganism to different management measures was discussed.It provides reference information for the management and sustainable development of alpine grassland.The main results of the study are as follows:(1)Under the fencing treatment,1647 OTU sequences were obtained in soil bacterial sequencing,82 OTU sequences were obtained from soil fungal sequencing,and 1657 OTU sequences were obtained in soil bacterial sequencing under grazing treatment,and 79 OTU sequences were obtained by soil fungal sequencing.(2)Under the fencing treatment,Proteobacteria and Chloroflexi were the dominant groups of soil bacteria Shared by MY,WY and YC.Ascomycota and Basidiomycota were dominant groups of soil fungi common to MY,WY and YC.On a genus level,Fusarium,Penicillium,Trichoderma and Cladosporium are dominant groups of soil fungi common to MY,WY,and YC.Under grazing treatment,the pH ?ylum community composition of soil bacteria and fungi in the three plots was the same as that under fencing treatment,and there was no significant difference.(3)At WY sampling point,soil bacterial and fungal abundance were significantly correlated with soil total carbon and total nitrogen contents.Soil bacteria and fungi were significantly correlated with soil moisture content and conductivity at MY sampling site.Soil bacteria and fungi were significantly correlated with pH ? and nitrate nitrogen at the YC sampling site.In the alpine grassland ecosystem under the fencing and grazing treatment measures,the differences of soil microorganisms in different niches were reflected in the abundance level of species,and there is no significant change in the composition of the community structure.