The Effect Of Bacterial Diet On Fat Storage In Caenorhabditis Elegans

Diet can significantly affect the health of the organism.The model organism Caenorhabditis elegans mainly uses Escherichia coli as a food source in laboratory,but the effects of different bacterial diets on the basic physiological characteristics of C.elegans are not fully clear.In this paper,four dietary Escherichia colis(OP50,MG1655,HT115,HB101)commonly used in the laboratory were used to feed C.elegans,and we investigated their effects on fat storage,developmental rate,reproduction and longevity in C.elegans.Then the signal pathways related regulating lipid storage were explored.In the study,we found that the bacterial diet significantly affected the lipid storage in C.elegans,and to some extent affected the development rate of C.elegans,but had no significant effect on fertility and longevity.Among them,the MG1655,HT115,and HB101 diets significantly reduced the lipid storage level in C.elegans compared with the laboratory standard diet OP50.Therefore,in order to explore the causes for the four E.coli diets affecting the lipid storage,we further studied the bacterial energy nutrient composition and the signal pathway related to regulating lipid storage in C.elegans.The results showed that there was no significant correlation between the difference in nutrient composition of the dietary diet and the level of lipid storage in C.elegans.Using lipid-metabolism-related mutants daf-2,aak-2,tph-1,and rict-1,we found that the higher lipid levels of OP50 compared to the other three bacterial diets may be related to the regulation of insulin signaling pathway.The AMPK and mTOR signaling pathways may mediate the lower lipid storage levels of HT115 compared to OP50.In summary,this study found that four E.colis mainly affect the fat storage in C.elegans,while insulin,AMPK and mTOR signaling pathways regulate the effects of four diets on the lipid storage C.elegans to different extents,which lays a good foundation for further exploring the detailed mechanism of bacterial diet regulating lipid storage and other physiological characteristics of C.elegans.