Preliminary Analysis Of ALDH7 And ALDH12 Gene Functions In Cotton Under Salt And Drought Stress

Plants live in complex environments.Due to the long process of evolution,plants have formed defense mechanisms to deal with biological and abiotic stress.ALDHs is extensively involved in plant responses to biotic and abiotic stress.In this research work,the functions of ALDH7?Gh_D06G1578?and ALDH12?Gh_D03G0856?genes of cotton ALDH gene family under drought and salt stress were preliminarity analyzed by using Arabidopsis thaliana transformation model.The result as follows:1.The expression level of ALDH7?Gh_D06G1578?in transgenic Arabidopsis thaliana was significantly higher than that in wild Arabidopsis thaliana under drought and salt stress.The activity of catalase?CAT?in transgenic plants was higher and the content of hydrogen peroxide?H₂O₂?was lower than that in wild-type Arabidopsis under drought and salt stress.The content of malondialdehyde?MDA?in the leaves of transgenic Arabidopsis was lower than that of wild-type plants,which indirectly indicated that the extent of lipid oxidation damage of transgenic Arabidopsis under stress was little.In addition,the expression levels of AtABF4,AtCBL1,AtSOS1 and AtRD29B in transgenic Arabidopsis were higher than those in wild-type plants under drought and salt stress.The results showed that Gh_D06G1578 could play a role in improve plant tolerance to drought and salt stress.2.Compared with wild-type,the seed germination rate and root elongation of ALDH12?Gh_D03G0856?transgenic Arabidopsis thaliana under ABA treatment were lower.Transgenic Arabidopsis is more sensitive to ABA and more susceptible to ABA regulation in response to stress damage.Compared with wild-type,transgenic Arabidopsis had higher CAT activity and lower H₂O₂ content.The MDA content of transgenic Arabidopsis was lower than that of wild-type plants,which indicated that the oxidative stress level of transgenic Arabidopsis was lower.Compared with wild-type,the leaves of Arabidopsis transgenic plants had higher relative leaf water content and lower excised leaf water loss rate and ion leakage.In addition,the expression levels of AtABF4,AtCBL1,AtSOS1 and AtRD29B in transgenic Arabidopsis were high under stress.The results showed that ALDH12?Gh_D03G0856?gene could play a role in improve plant tolerance to drought and salt stress.