Synthesis And Properties Of Polarity Fluorescent Probes For Targeting Mitochondria

Polarity is an important parameter in the chemical and chemical fields.Polarity has been studied intensively as a key parameter affecting chemical reactions.At the same time,the polarity problem in the biological micro-environment has also received widespread attention.Different physiological processes and pathological processes correspond to different cellular states,cell polarity-the morphological and functional differentiation of cellular compartments in a directional manner is required for processes such as orientation of cell division,directed cellular growth and motility.When the state of the battery changes,the polarity changes to some extent.Therefore,detecting changes in intracellular polarity is critical to monitoring different cellular states and can help us gain insight into physiological processes and pathological processes.Many of the key questions facing cell biology involve the location and concentration of chemicals,from molecular signals to metabolites to exogenous toxins.Fluorescence molecular imaging is an indispensable tool in the fields of biology and life sciences,fluorescent probes have attracted widespread attention due to their high sensitivity,strong selectivity,fast response,and simple operation.They have become an important tool for monitoring the microenvironment in cells.Mitochondria,as essential and interesting organelles within the eukaryotic cell,play an important role in maintaining numerous functions in human physiology.The fundamental role of mitochondria is to produce energy through oxidative phosphorylation and lipid oxidation.Besides this well-known function as power factories,mitochondria are also believed to be,in a sense,nerve centers for controlling the cell in certain aspects.Mitochondrial polarity is a crucial characteristic of organelles,and tremendously impacts cellular events.Mitochondrial polarity affects many events,including protein transport and interactions,enzyme activity and stability,and maintenance of cellular function and homeostasis.Therefore,accurate tracking of mitochondrial polarity is of great significance.In this study,we read a large amount of literature to design and synthesize the fluorescent probe Mito-DF.Coumarin has solvent-chromic properties and is used as a fluorescent group of the probe Mito-DF,which has excellent mitochondrial targeting and is used as a probe Mito-DF mitochondria targeting group.MPP is attached to one end of the coumarin skeleton as an electron-donating group,and the other end is linked to methoxyphenylacetylene as an electron withdrawing group to form a"push-pull"electronic structure.The probe Mito-DF has a good conjugated structure,showing good two-photon performance,the probe has a large Stokes shift in response to polarity,and can provide a good two-photon fluorescence detection signal.At the same time,the coumarin is used as the fluorescent group,which makes the fluorescent probe Mito-DF have longer absorption and emission wavelength regions,higher two-photon absorption characteristics,lower biological toxicity,better water solubility,and the like.Properties are very advantageous for bioimaging applications.More importantly,the molecular design of the fluorescent probe Mito-DF is very sensitive to changes in environmental polarity.Various advantages indicate that Mito-DF can be used as an excellent two-photon fluorescent probe to detect environmental micropolarity.The change.We used two-photon fluorescence microscopy to successfully detect changes in micropolarity in the mitochondria by changes in the fluorescence of the probe Mito-DF.