Effect Of Dicranopteris Dichotoma In The Process Of Vegetation Restoration On Soil Microbial Community And Diversity In Red Soil Erosion Area

Red soil erosion area in Subtropical China is the serious soil erosion area and fragile ecological environment.Ecological restoration of soil and water conservation in recent 30 years,the gradual recovery of vegetation,forest biomass growth,improve soil fertility,but Dicranopteris dichotoma in the process of vegetation restoration change on soil microbial community structure and influencing factors of soil microbial lack of understanding.In this study,the eroded land of red soil in Hetian Town,Changting County,Fujian province is selected as the research area,and without building measures of vegetation restoration(YO),13 years of vegetation restoration(Y13)and 31 years of vegetation restoration(Y31)were selected as experimental sites.We implemented three kinds of treatment(NRd-Not removed Dicranopteris dichotoma;Rd-Removed Dicranopteris dichotoma;CK-Control)at experimental plots.The bacterial 16S rDNA and fungal ITS were sequenced using Illumina Miseq sequencing technology.The results showed that:(1)The effect of vegetation restoration and Dicranopteris dichotoma on soil moisture is little,The value of soil pH decreased with the vegetation restoration and Dicranopteris dichotoma.The concentration of soil organic carbon,total nitrogen,total phosphorus,available phosphorus,soil carbon and nitrogen ratio,ammonium nitrogen and soil microbial biomass increased significantly with vegetation recovery;the increase in recovery years has a significant effect on ammonium nitrogen and soil microbial biomass carbon;the content of nitrate nitrogen is low to high with the restoration of vegetation.Dicranopteris dichotoma lead the contents of soil organic carbon,total phosphorus,soil carbon nitrogen ratio,instant phosphorus and soil microbial content increased respectively,which reduced the nitrate content.(2)A total of 1710503 optimization sequences of soil bacteria were obtained,and soil bacterial communities were identified as 42 phylums,100 classes,209 orders,387 families,700 genera,1232 species and 4987 OTUs respectively;the optimum sequence of soil fungi is 1675385,and fungi Community has 6 Phylums,24 classes,72 orders,162 families,293 genera,453 species and 1544 OTUs.The amount of sequencing data is reasonable and can reflect the diversity and species of the vast majority of soil microbial communities.The richness of soil bacterial community increased significantly with the increase of vegetation restoration years(P<0.05),bacterial diversity increased with vegetation recovery but restoration years had no significant impact on diversity;the richness and diversity of the fungal community also increased significantly with the restoration of vegetation(P<0.05),but the recovery years had no significant effect on the richness and diversity of fungi.This shows that the diversity of soil microbial community is stable in a certain time,and the richness of fungi is more stable than the richness of bacteria.Dicranopteris dichotoma in the control has a significant influence on the richness of soil microbial;the control sample of the fungal diversity increased significantly,had no significant effect on bacterial diversity.Dicranopteris dichotoma in the control plots more significant effect on soil microbial,while the impact of the fungal diversity is greater than bacteria.Soil microbial diversity of Beta showed that vegetation restoration and Dicranopteris dichotoma have significant effect on soil bacteria and fungi.The results of redundancy analysis showed that soil pH,soil organic carbon,total nitrogen,total phosphorus,carbon and nitrogen ratio,available phosphorus,nitrate nitrogen,ammonium nitrogen and aboveground litter were the influencing factors of microbial community richness and diversity,among them,total nitrogen,available phosphorus and aboveground litter are the main influencing factors.(3)The level of soil bacterial community relative abundance is greater than 1%,which are Proteobacteria,veronicus,actinomycetes,Actinobacteria,floating mold,posterior wall,verruca,microphylum,GAL 15,Bacteroides and Thaumarchaeota,accounting for more than 97%of the total number of soil bacteria.Vegetation restoration increased the relative abundance of Proteobacteria,Actinomycetes,Actinobacteria,Planctomycetes and Verrucomicrobia,reduced the relative abundance of Chloroflexi,Firmicutes,GLA15,Bacteriomonas and Thaumarchaeota.The Phylum level of soil fungal community are Ascomycota,Basidiomycota,Zygomycota and Chytridiomycota,the dominant fungal of phylum level are Ascomycota,basidiomycota.The vegetation restoration and Dicranopteris dichotoma have no significantly influence on the relative abundance of Ascomycota,and the relative abundance of Basidiomycota decreased significantly with Dicranopteris dichotoma.Redundancy analysis showed that aboveground litter and available phosphorus content made the main influencing factors of soil microbial community.