Study On The Sulfur Oxidation Of AMDY2-9-2 Based On Whole Genome Technique

AMDY2-9-2 was isolated from acid mine drainage in Tong Ling,Anhui Province The environment there is featured with iron-sulfur compounds contained,high concentration of heavy-metal,low pH and so on,which leads to the acid mine drainage environment(AMD).The formation of AMD is not only caused by the natural oxidation of sulfur-containing compounds through chemical processes,but also by the biological effects of some sulfur-oxidizing microorganisms.This study is focused on the capacity of sulfur-oxidizing of AMDY2-9-2 based on whole genomic analytic technique and a series of physiological-biochemical experiments.With the De-novo whole genome technique,the whole genomic sequences of AMDY2-9-2 were gotten and then annotated by database such as GO,COG,KEGG and so on,which helps us to find genes related with sulfur metabolism and their possible sulfur metabolic way.At the same time,by the comparative genomic analysis with model sulfur-oxidizing microorganisms,some idea about how AMDY2-9-2 work when sulfur oxidation happens was obtained.According to the results of whole genomic technique,then,some physiological and biochemical experiments are followed to verify the capacity of sulfur-oxidizing of AMDY2-9-2.0,1,3,6,9g/L and 1g/L different particle size element sulfur were separately added into FeT culture medium to study the sulfur-oxidizing of AMDY2-9-2.In addition,the route of exposure to the element sulfur and the electron acceptor in FeT was changed to study how AMDY2-9-2and its protein functioned.All these efforts are aimed to find the key step of sulfur oxidation in AMDY2-9-2,so that the processes of sulfur-oxidizing and the AMD can be controlledAccording to the whole genomic technique results,AMDY2-9-2 has its own sulfur-oxidizing system but no obvious independent iron-reducing system,but genes related to iron metabolism is clustered as Fe-S complexes,which means iron metabolism may be related with sulfur metabolism processes.In addition,one gene can be find in several metabolism processes,which means SO42-and Fe2+ that we considered as the indication of sulfur-oxidizing and iron-reducing might be the production of other metabolism processes.So,physiological and biochemical experiments are necessary to verify the sulfur and iron metabolism process.In the physiological and biochemical experiments,the growth of AMDY2-9-2,pH,Eh,SO42-and Fe2+ concentration were detected as the marker of sulfur oxidation and iron reduction.Then,several results were obtained.First of all.when the concentration of S0? 6g/L,the sulfur-oxidizing of AMDY2-9-2has positive correlation with the S0 concentration,and with the particle size of S0 be smaller and smaller,the sulfur-oxidizing be faster.The study of mechanism of sulfur oxidation shows that only active AMDY2-9-2 touch S0 directly or indirectly,can oxidize S0,and during this period,O2 and Fe3+ both can be the electron acceptor,but O2 is the more effective one,which suggests sulfur-oxidizing and iron-reducing can be a couple process when there is lack of O2 in the environment.