Self-assembly Of Biomacromolecules And Inorganic Nanoparticles

There are extensive interactions between biomacromolecules and inorganic nanoparticles in nature.From pearl coral to human bones and teeth,all are the result of the interaction between biological macromolecules and inorganic nanoparticles.Compared with macroscopic materials,inorganic nanoparticles have many unique optical properties,such as small size effect,quantum confinement effect,macroscopic quantum tunneling effect and so on.Biomacromolecules(such as DNA proteins)are highly ordered,and their self-assembly size can be adjusted.Moreover,functional groups can be modified on biomacromolecules,which can be synthesized under human intervention.The interaction between biomacromolecules and inorganic nanoparticles is bound to generate a series of interesting structures which have been widely used in the field of optical and electrical nano-devices or cancer treatment.The interaction between DNA and gold nanoparticles,silver nanoparticles or silver nanoparticles was studied in this paper.At the same time,interactions between inorganic nanoparticles and Amyloid fibers were explored and a series of characterization methods were used for their self-assembled products.The main research content of this paper is as follows:(1)Sodium citrate ligand exchange method was used to successfully functionalize 13nm gold nanoparticles with DNA within 2h,and we made a series of explorations on the reaction conditions of the modification.Finally,we self-assembled the gold nanoparticles modified by this method with the triangular DNA Origami,and obtained the assembly of gold nanoparticles and DNA Origami.(2)The sodium citrate ligand exchange method was successfully applied in the silver nanoparticles modification.At the same time,we explored the conditions of the modification of silver nanoparticles.It was found that under the protection of sodium dodecyl sulfate(SDS),the DNA functionalization of silver nanoparticles could be successfully completed within 80 min by using Na2SO4 as aging salt.In the end,we assembled the modified silver nanoparticles with octahedral DNA Origami,formed the structure of silver nanoparticles-DNA Origami.(3)On the basis of the experience of modifying silver nanoparticles,we applied this method to the modification of silver nanoprisms,and obtained the silver nanoprisms with DNA functionalization,and the silver nanoprisms with DNA modification has the induced chiral circular dichroism(CD)peak.(4)The interaction between inorganic nanoparticles and Amyloid was explored.By means of self-assembly of gold nanoparticles and silver nanoparticles with blue InP@ZnS QDs,a series of inorganic nanoparticle chains with highly ordered arrangement were obtained.Moreover,optical properties of some samples were characterized.In summary,in this paper,by using the sodium citrate ligand exchange method as the basic method,the DNA functionalization of gold nanoparticles,silver nanoparticles and silver nanoprisms was completed in a short time mainly using multi chimeric phosphorothioate modified DNA(ps-po-DNA).Moreover,we have completed the self-assembly of gold nanoparticles,silver nanoparticles with DNA Origami,and we also have completed the self-assembly of various inorganic nanoparticles with amyloid,thus obtaining a series of self-assembly aggregates,which have potential application values in optics,electronics,medical imaging and other fields.