Study On The Expression Of Auxin Response Genes And Temperature Stress Responsive Genes In Sorghum And Arabidopsis

Temperature is one of the important environmental factors that affect plant growth.In recent years,because of the changes in global climate and environment,many plants have been affected by temperature stress.In order to explore the molecular mechanism of the effect of temperature stress on plant growth and development,the expression patterns of ARFs(Auxin Response Factors)in normal plants and plants after temperature stress were studied in Sorghum.For further study,we changed Sorghum into Arabidopsis,which has more clearly genetic background.Then,based on the morphological observation,the possible stress responsible genes,especially response in auxin and other hormones signaling pathways,were explored under the temperature stress condition.Main results are described as follows:1.By using bioinformatics method,25 ARF genes were screened in Sorghum(Sorghum bicolor).The expression of the 25 ARF genes in different tissues of sorghum was studied by qRT-PCR.In addition,temperature stress experiments on Sorghum seedlings were conducted,and some temperature stress responsible ARF genes were found.Then five high expression ARF genes were selected,their localization and characterization were studied by in situ hybridization.2.The three dimensional reconstruction and observation of Arabidopsis after high temperature stress were carried out by the Optical Projection Tomography(OPT).It was found that the high temperature stress had a serious effect on the growth and development of the ovules in Arabidopsis,but the effect of low temperature was not obvious.In order to investigate the high temperature responsible genes of Arabidopsis,transcriptional sequencing technology was used.48 G gene expression data was obtained,and bioinformatics analysis was carried out on the sequencing data.3.In order to analyze the differentially expressed genes data in the transcriptional transcript(TT)better and further screen the genes that respond to high temperature stress during the development of the ovule of Arabidopsis.On the one hand,a comparative analysis of plant hormone signal transduction pathway(PHST)in KEGG(Kyoto Encyclopedia of Genes and Genomes)database and the TT data was carried out.It wasfound that there were 42 gene families in the TT data that accounted for 270 genes in the PHST(71 of them were significant differences before and after high temperature stress),and made the heat map of genes related to the high temperature stress of Arabidopsis in PHST.Then the auxin signal pathway was selected to verify the expression of 20 auxin regulating genes which were significantly expressed in the TT database by real time quantitative PCR analysis.The results showed that 15 genes in Arabidopsis responded to the high temperature stress of the pre differentiated ovule(embryonic development to the spherical stage),and 5 genes responded to the differentiation after differentiation.High temperature stress on ovule(embryo development to torpedo stage).On the other hand,44 functional genes,which are closely related to Arabidopsis and high temperature stress,are compared and analyzed in the TT database in the last 5 years,and 16 genes with significant differences are screened.Real time quantitative PCR analysis showed that of these 16 genes,9 genes responded to the high temperature stress of the ovule before differentiation,and 10 genes responded to the high temperature stress of the ovule after differentiation.