Microfluidic Platforms For Cell Separation,Single-cell Encapsulation And Cell Proteomics Analysis

Recent years have seen great development in the field of fundamental biological and clinical research,which put great demands on the development of new,advanced methods for cell separation and cell analysis.Cell separation,especially the separation of rare cells,plays an important role in relevant research and applications.Continuous and droplet-based microfluidics,whose dimensions are comparable with cells,permit accurate spatial control of cells in the length scale of nano-and micrometers using geometries and externally applied force fields.So far,they have been broadly used in the isolation of cells,single-cell encapsulation and cell analysis in the scale of molecules.In this thesis,we developed microfluidic platforms for isolating rare cells and for determined single-cell encapsulation.Based on droplet microfluidic techniques,we synthesized highly mono-dispersed SiO2 particles and built up a platform for proteomics analysis of target cells.The outline of this thesis is as follows.Chapter 1 gives detailed introduction on cell separation and cell analysis,which includes the importance of cell separation and single-cell analysis,available methods and techniques for isolating cells,microfluidic single-cell analysis and cell proteomics.Chapter 2 details the design and fabrication of cell-separation chips,which are based on inertial separation of cells in curved channels.Using mono-dispersed polystyrene microsphere,the separation effects of our microchips are performed.Chapter 3 introduces the determined single-cell encapsulation microchip we have designed and fabricated,which achieved single focusing of randomly distributed PS particles and single-particle encapsulation with high efficiency.Chapter 4 shows the target cell proteomics analysis platform we have built,and the analysis of ca,1000 K562 cells gives out 143 protein identified.Chapter 5 summarizes the studies and gives outlook to the future research.