Investigating The Role Of Fabp11a In Brain Vessels During Zebrafish Development

Objective: Investigating the role of fabp11 a during brain vessel development in zebrafish.Methods: 1.Using bioinformatics software Jalview and Phy ML to study the conservation and evolutionary relationships of fabp11 a among different species.2.Using revers transcript polymerase chain reaction(RT-PCR)and whole mount in situ hybridization to explore the temporal and spatial expression in developmental zebrafish.3.Analyzing the role of fabp11 a in vascular system.(1)We constructed fabp11 a mutant using CRISPR-Cas9 system and observed the phenotypes,and we knock down fabp11 a to confirm the phenotypes.(2)Coinjection fabp11 a m RNA in fabp11 a mutant can rescue the phenotypes.4.Analyzing the molecular mechanisms of fabp11 a in developing zebrafish cerebrovessels.Results: 1.Fabp11 a is highly conserved across vertebrates.2.Fabp11 a is expressed in the developing zebrafish brain vessels3.Fabp11 a loss-of-function caused hemorrhage and impairs brain vessel integrity4.Macrophages migrated into zebrafish brain in fabp11 a mutant5.COX and LOX inhibition partially rescued the brain vessel integrity defects caused by fabp11 a loss-of-functionConclusion: Fabp11 a plays a key role during brain vessel development and regulates the brain vessel integrity through COX and LOX signaling pathways.