Preparation Of Lecithin Doped Electropun Polylactic Acid-polyurethane Nanofibers And Its Effect On Hepatocyte Viability

The liver controls and affects almost every aspect of human metabolism and physiological regulation and is an important internal organ in the human body.With the improvement of people's living standards and the acceleration of the pace of life,various kinds of liver diseases have emerged and become one of the major diseases that plague human.At present,liver transplantation is the only treatment for end-stage liver disease and liver failure,but many problems such as shortage of donor organs,high cost,and immune rejection make it impossible to effectively and quickly resolve liver disease problems.In order to seek a better solution,scholars have long dedicated to the development of artificial liver support systems(ALSS)and bioartificial liver support system(BLSS)in vitro as an alternative to whole organ transplantation.However,in BLSS,the viability and function of hepatocytes directly affect the performance of the bioreactor.Three-dimensional(3D)scaffold cell culture has attracted extensive interest from researchers as an effective method for hepatocyte culture in vitro.As a commonly used method for preparing 3D scaffolds,electrospinning technology is simple in operation,easy to control,and low in cost.Fibers obtained by electrospinning have ultra-high specific surface area,adjustable porosity and are easily processed into various shapes.In addition,they have interconnected porous structure that mimics extracellular matrix(ECM)well,which is conducive to cell communication,cell adhesion,and nutrient delivery.Therefore,electrospinning technology has received extensive concern and study in tissue engineering and regenerative medicine.In this study,a synthetic polymer thermoplastic polyurethane(TPU)with good mechanical properties and flexibility was blended with polylactic acid(PLA),which lacks mechanical strength and flexibility,and lecithin(Lecithin,Lec)was added to combine excellent properties of natural polymers and synthetic polymers.PLA-TPU-Lec composite nanofibers with improved mechanical properties,wettability and cell compatibility were prepared.The viability of hepatocytes cultured thereon is improved,and it is expected to be used for hepatocyte culture in vitro to improve cell viability in artificial liver bioreactors.The results obtained in this paper by experimental research are as follows:1.PLA-TPU composite nanofibers were prepared by using electrospinning technology and adjusting the parameters of each process.2.The PLA-TPU-Lec composite nanofibers with good morphology were prepared by introducing lecithin into PLA-TPU through blending modification.The results show that the diameter and hydrophobicity decreased with the increase of lecithin.3.Cellular experiments showed that hepatocytes(HepG2 cells)adhered and grew we ll on the surface of the composite nanofibers,and cell proliferation was evident.Among them,the PLA-TPU-Lec(5%)composite nanofibers obtained through experimental screening have the best cell compatibility.4.The PLA-TPU-Lec(5%)nanofibers were used to culture HepG2 cells,and HepG2 cells were cultured in tissue culture dishes(TCP)as controls.Hepatotoxic toxicants were added both.After incubation,it was found that the activity of HepG2 cells in the PLA-TPU-Lec(5%)nanofiber culture model was significantly higher than that of the TCP culture model.