Isolation And Identification Of A Deinococcus,Carotenoid Purification And Optimization Of Fermentation Conditions

The development and utilization of radiation-resistant strains and revealing their radiation resistance mechanism are of great significance in environmental protection,bioremediation,and human health.In this experiment,one strain was isolated from the water samples from the grassland Silk Road culture theme park in Hohhot.The16 S r DNA sequence was PCR amplified and aligned to GenBank database Blast,showing 100% similarity to the Deinococcus aquaticus strain 35.By observing the morphology of colonies,combined with physiological and biochemical experiments and UV radiation resistance tests and molecular identification results,the stain is finally classified to a species of Deinococcus.The purpose of this research was to study the pigment produced by the fermentation of Deinococcus spp.,the extraction conditions of the strain were optimized so as to increase the yield of the pigment.After the bacteria were obtained through fermentation,the pigment was extracted from cell wall and purified using silica gel plate chromatography to obtain three types of pure pigments.A full-wavelength scan of the pigment extracted from the strain was performed by a multi-functional microplate reader to determine the carotenoids production of three different colors.The Gram stain test results showed that the strain was Gram-positive bacillus,the optimum growth temperature was 32°C,and the optimum growth pH was 7.The UV radiation resistance test of this strain of Deinococcus and E.coli showed that the strain has strong radiation resistance.The isolation and identification of radiation-resistant strains of Deinococcus.and to study its characteristics have certain significance and value for understanding the radiation-resistant mechanism of radiation-resistant strains.