The Differences Of Two Spectral Analysis Methods Of Single-voxel And Multi-voxel In The Hippocampus And Thalamus Data

Objective To investigate the single-voxel and multi-voxel magnetic resonance spectroscopy in hippocampus and thalamus of healthy volunteers,and to explore whether the data obtained by the two methods are statistically different.Methods Use the single-voxel and multi-voxel MRS detection and analysis bilateral hippocampus and thalamus of the same age in 50 healthy adult,which N-acetyl aspartic acid(NAA)/creatine(Cr),choline(Cho)/creatine(Cr),NAA/(Cho+Cr)ratio can obtained.Results there were significant differences in Cho/Cr and NAA/(Ch+ Cr)in the left hippocampus between single-voxel and multi-voxel MRS(t=-5.239,4.042,P<0.05);NAA/Cr values were not statistically significant(P>0.05);NAA/Cr and Cho/Cr values in the right hippocampus were significantly different between single-voxel and multi-voxel MRS(t=-3.491,-3.769,P<0.05).There was no statistically significant difference in NAA/ Cho+Cr(P>0.05);The NAA/Cr and Cho/Cr values of single-voxel and multi-voxel MRS were statistically different(t=-2.552,-6.273,P<0.05),and there was no significant difference in NAA/(Cho+Cr)(P>0.05).NAA/Cr,Cho/Cr,and NAA/(Cho+Cr)(t=-5.862,-5.068,-6.316,t=-11.722,-9.077,-9.712,t=-11.314,-9.379,-10.842,P<0.05)were statistically difference in the left thalamus,right thalamus,thalamus with single-voxel and multi-voxel MRS.The NAA/Cr,Cho/Cr,and NAA/(Cho+Cr)levels in the left and right hippocampus of the single-voxel MRS were statistically significally(t=4.369,-5.073,6.959,P<0.05).The NAA/Cr,Cho/Cr,and NAA/(Cho+Cr)levels in the left and right hippocampus of the multi-voxel MRS no statistically significant.(t=1.191,1.503,-0.133,P>0.05).There were no significant difference in NAA/Cr,Cho/Cr,NAA/(Cho+Cr)in Left lateral thalamus with single-voxel MRS(t=1.191,1.503,-0.133,P>0.05),There were no significant difference in NAA/Cr,Cho/Cr,NAA/(Cho+Cr)in Left and right lateral thalamus with multi-voxel MRS(t=-1.141,-0.881,-0.252,P>0.05).There were no significant difference in NAA/Cr,Cho/Cr,NAA/(Cho+Cr)with singlevoxel MRS in normal hippocampus of different genders(t=-1.960,0.122,1.866,P>0.05).There were no significant difference in NAA/Cr,Cho/Cr,NAA/(Cho+Cr)with Multivoxel MRS in normal hippocampus of different genders(t=1.210,-0.493,1.661,P>0.05);there was a statistical difference between Cho/Cr and NAA/(Cho+Cr)in thalamus of different sexes in normal individuals with single-voxel MRS(t=-2.194,2.795,P<0.05),NAA/Cr values were not statistically different(t=1.112,P>0.05);there was a statistical difference between Cho/Cr in thalamus of different sexes in normal individuals(t=-3.27,P<0.05),NAA/Cr and NAA/(Cho+Cr)values were not statistically different with multivoxel MRS(t=-0.580,1.722,P>0.05).Conclusion The single-voxel and multi-voxel MRS are two independent examination methods,and the detection values obtained for the same area of interest are not the same.Therefore,their values cannot be used universally.When the single-voxel MRS was examined,there were inconsistencies between the left and right sides of the normal hippocampus.The study of single-voxel and multi-voxel magnetic resonance spectroscopy on normal human hippocampus is not affected by gender,while the study of the thalamus is influenced by gender.