Preliminary Study Of Force Driven Particle Sorting Methods On Microarray

Particle sorting is an important research method in the field of bioengineering,it is often used as the basis for single cell analysis,molecular diagnostics and other research.Among many sorting devices,microfluidic sorting chips based on microarray sorting technology become the research hotspot in cell biology and biotechnology for low cost,easy manipulation,simple structure,and small cell damage.Aiming at the deficiencies of directional locking technology and the separation theory in the field of numerical simulation and practical application,simulation analysis and experimental research were carried out in this study.Firstly,related theories of different microarray cell sorting methods were compared and analyzed,on which basis COMSOL Multiphysics 5.3 was used to establish a two-dimensional microarray sorting structure model corresponding to the directional locking and separation of periodic microarrays.In the model,the flow field and particle trajectory were solved by computing the wall distance and related modules,and the influence of external force F and micro-array structure parameters on the motion of particles was discussed,which guided the following design of microchip and the experimental operation.Secondly,from the above theory,a centrifugal disc microarray sorting chip was designed to explore the sorting situation of the microarray chip driven by centrifugal force.On the preparation of the chip,the photolithography template method and the oxygen plasma treatment method were respectively used to complete the preparation of the microchannel of the chip and the sealing connection of the microchannel.According to the specific needs of the experiment and combined with the specific characteristics of the chip,the related instruments and equipment were used to construct the points.The experimental platform was selected,and polystyrene standard particles with diameters of 9.7 ?m and 37 ?m were selected as experimental objects to verify the sorting performance of centrifugation microarray cell sorting.Successful separation of the two types of particles was performed.And based on this,the effect of the tilt angle of the microarray on the sorting results was explored.The experimental results were consistent with the theory of directional locking and separation of the periodic microarray.In order to improve sorting efficiency,the micro-column shape of the chip were also improved,and a centrifugal disc triangular micro-column array sorting chip was designed and developed.However,using the same experimental method,the experimental results show that the sorting efficiency has not been significantly improved..Finally,in order to improve sorting efficiency and observability of the experimental process,the sorting experiments of microarray sorting chips driven by centrifugal force was comprehensively optimized,and a magnetic field force microarray sorting chip was designed and developed.Silica magnetic microspheres with diameters of 10 ?m and 40 ?m were used as experimental objects to verify the sorting performance of the separation chip.Based on the successful implementation of sorting,the influence of the tilt angle of the microarray on the sorting effect was explored.The experimental results were consistent with the theory of directional locking and separation of periodic microarrays.Compared with centrifugal disk microarray sorting chips,the sorting efficiency was improved overall.In summary,the research work carried out in this study not only provides simulation basis for the theory of directional locking and separation of periodic microarrays,but also further expands the theoretical application range and realizes a new microfluidic particle sorter,it lays a good foundation for the research work on biological cell sorting to be carried out later.