The Network Of Salt Tolerance Genes Of Cercis Gigantea Was Constructed Using High-Throughput Sequencing

Salt stress is one of the main environmental factors that affect the growth and development of plants.An in-depth understanding of the mechanism of salt stress helps to study the salt tolerance of plants.Many substances are involved in the regulation of plants in response to salt stress.MicroRNAs(miRNAs)play an important role in the regulation of salt stress.If we can deeply understand the regulation mechanism of miRNAs and construct a regulatory network of plant genes that are highly salt tolerant,it will provide an important theoretical basis for improving the plant tolerance to high salinity.The Cercis gigantea,as an extensive greenery species,has its own weak salt tolerance.If it can improve the salt tolerance,it will make it an excellent greenery plant in the saline area.Unfortunately,there is no any study on salt resistance of Cercis gigantea.In this paper,we use the genome sequencing,transcriptome sequencing and miRNA sequencing technology to study the Cercis gigantea under salt stress conditions,identify the miRNA and its target genes,and build a regulatory network of genes related to high salt tolerance of the Cercis gigantea.In order to study the expression level of miRNAs under high salinity conditions in Cercis gigantea,we used 200 mM NaCl solution for 48 h,and then extracted the total RNA from the roots and leaf tissues of Cercis gigantea for miRNA sequencing.Through the processing of sequencing data,407 miRNAs of 70 miRNA families are known to be identified.Among them,there are 80 miRNAs that are significantly differentially expressed under salt stress,such as the miR160 and miR171 families.Next,predicting miRNA target genes by software,19787 miRNA-related target genes were identified.By annotating the function of differentially expressed miRNA target genes,we found that a part of the target genes involved in the stress response signal pathway and the regulation of cell membrane transporters.The multi-omics study of Cercis gigantea includes the construction of genome and the assembly of transcriptome.Since the genomic data of Cercis gigantea have not been published so far,and the assembly of transcriptome lacking the reference genome will generate large errors,the genome sequencing of Cercis gigantea was performed for the first time.During the process of assembling the genome,Cercis gigantea was found to be a complex genome.Therefore,we used multiple genome assembly softwares to ensure the accuracy of genome assembly.Afterwards,the transcriptome was sequenced from Cercis gigantea.Using the traditional method of assembly with reference genome,44148 Cercis gigantea expressed transcripts were identified.Among them,69 genes showed significant differences by differential expression analysis.Finally,the NCBI database was used to find out the plant salt tolerance genes and compose the genes datasets.Secondly,we used the protein interaction network data of soybeans to compare the transcripts of Cercis gigantea using methods of near-source research to find the homologous proteins with higher matching and build the protein interaction network of Cercis gigantea.Using the same idea,the significantly differently expressed target genes of miRNA and transcripts and plant salt resistance gene datasets were linked into the protein interaction network of Cercis gigantea,and eventually constituted the regulatory network of Cercis gigantea salt tolerance-related genes.The results of this thesis not only provide the theoretical basis for the breeding Cercis gigantea of high salinity tolerance varieties,but also provide new ideas and methods for the study of salt tolerance of legumes.