Treatment Of Acid Mine Wastewater With Sulfate Reducing Bacteria As The Core

With the development of industry,which resulted in the form of acid mine drainage water with low p H,multiple heavy metal ions,and high sulfate concentr ation in the production of mining.The study of the use of Sulfate-Reducing Bacteria（SRB）in the microbial process for the treatment of AMD has low expense,and it does not have secondary pollution.This article clarifies the SRB’s tendency to catabolize sulfur metabolism in the process of sulphate metabolism,and provides a theoretical basis for the metabolic regulation of sulfur ions in the target product.On this basis,according to the characteristics of AMD,the SRB is used to perform electrol ytic pre treatment before processing AMD,and then SRB is used for processing.The SRB integrated reactor is designed to explore the changing rules of metal ions,sulfate and the like in the process.The main results are as follows:（1）Under the condition of constant voltage,the effect of Fe-Fe plate electrol ysis pretreatment on AMD is the best.The output of p H and hydrogen in wastewater increases with the increase of voltage.The removal rate of manganese ion,zinc ion and copper ion showed an upward trend with the increase of voltage,and the removal rate of copper ion was the highest.The XPS anal ysis shows that manganese mainl y exists in two valence state and zero valence state.The valence state of copper is one price or zero price.XPS anal ysis shows that coppe r exists in the form of two valence in addition to one valence state,and zinc exists mainl y in the form of zero valence and two valence state,and the iron valence state is trivalent.（2）The rate-limiting step during the deoxidation of SRB was mainl y the conversion of sulfite to hydrogen sulfide.When nitrogen was blown to desulfurize hydrogen,the amount of sulfur ions increased by 2.36 times,and the removal rate of sulfate increased from 79.63%to 87.45%.The sulfite and thiosulfate concentrations were decreased.In the process of SRB reduction,nitrogen was blown into the hydrogen sulfide,the p H value increased significantl y,and finally reached 8.31,while the final p H value of the reactor without blowing hydrogen sulfide was 6.87;during the SRB metabolism,sulfite and sulfide were produced.Metabolites other than hydrogen reduce ORP.The main microbial populations present in the two reactors are Desu lfo vi bri o,C o ma mo nas,Ps eud o mo nas,among which the Desu lfo vi bri o are the dominant bacteria in the two rea ctors.After nitrogen is blown to desulfurize hydrogen,the dominant bacteria are relativel y Abundance is high.It shows that the presence of hydrogen sulfide inhibits the proliferation of dominant bacteria to some extent.（3）The pretreated AMD is added t o the SRB integrated reactor,indicating that increasing the p H of the solution during the action of hydrogen sulfide and wastewater is more conducive to the removal of metal ions.When SRB was treated with sodium lactate as carbon source and electron dono r,the removal rate of sulfate was the highest,and the sulfate removal rate was 81.65%after 24 hours.With the increase of reaction time,the overall p H value showed a trend of first decrease and then rise to a stable trend.The AMD wastewater after blow ing hydrogen sulfide reaction was filtered and added to the SRB reaction s ystem.The removal rate of Fe²⁺was 87.62%,the removal rate of Zn²⁺was 82.91%,and the removal rate of Mn²⁺was 66.25%.The microbial communit y identification of strains showed tha t Desulfovibrio was the dominant genus,accounting for 69%of the entire bacterial communit y in addition.After the acclimation of SRB with s yngas as carbon source and electron donor,it was found that the final removal rate of sulfate in the 96-hour reaction was 77.86%.The p H value showed a trend of decreasing first and then increasing with time.ORP decreased during the whole reaction and then stabilized.There were two main dominant bacteria,Desu lfo vi bri o and Th iob acill us in the microbial communit y.Desulf o vib rio accounted for 48%of the entire bacterial communit y,and Thiobacillus accounted for 43%of the entire microbial community.