Regulation Of Glandular Trichome Development By MYB Transcription Factor MYB2 In Artemisia Annua L.

Artemisinin is an important active component of Artemisia annua L.,a medicinal plant,and it is an effective drug for malaria treatment.At present,artemisinin has achieved chemical total synthesis and genetic engineering biological semi-synthesis,but because of the restriction of substrate,catalytic enzyme and yield,neither of these methods has been used in industrial production.Artemisinin was mainly derived from Artemisia annua L,and artemisinin content in Artemisia annua L.was low(0.01-1.09%).It is of great significance to explore how to increase the content of artemisinin in Artemisia annua L.Glandular trichomes is a tiny protruding structure on the leaf surface of Artemisia annua L.It consists of 10 cells,namely,two basal cells,two stalks,two stalk cells,four sucking cells,and two apical cells.Glandular trichomes is a barrier against external stress.In addition to resistance to external stress,glandular trichomes is also a site for artemisinin synthesis and storage,and some key enzyme genes associated with artemisinin synthesis are also present in Artemisia annua L.glandular trichomes.MYB transcription factor is a class of transcription factors with myb binding domain.It is reported that a large part of MYB transcription factors have the function of regulating the development of trichome.This paper studies the regulatory function of myb transcription factors and the development of Artemisia annua L.glandular trichomeses.Firstly,the MYB2 gene was screened and cloned from the transcriptome library of Artemisia annua L.The gene was analyzed by bioinformatics,gene characterization and protein structure and function prediction.It was found that the gene belonged to MYB transcription factor.By subcellular localization and gene interaction experiments,it was proved that MYB2 protein was located in cell membrane and nucleus,which was consistent with transcription factor localization.At the same time,it was found that MYB2 protein could directly interact with Arabidopsis glandular hair regulatory complex(GL3)and participate in the regulation network of glandular hair development.Then,we constructed Arabidopsis thaliana plants with overexpression of MYB2 gene,and found that MYB2 gene can regulate glandular hair development.The function of biomass and anthocyanin synthesis.On this basis,we explored the metabolic engineering strategy based on MYB2 gene,constructed MYB2 gene overexpression and silencing transgenic plants of Artemisia annua L.,and found that MYB2 gene can not only negatively regulate the growth and development of glandular trichomes.It can also regulate the expression of key enzyme genes in artemisinin biosynthesis and affect the biosynthesis yield of artemisinin simultaneously.In conclusion,it is feasible to improve the yield of artemisinin biosynthesis based on the MYB2 gene metabolic engineering strategy.It has important guiding significance and application value for the cultivation of artemisinin high yield plants.