Preparation And Biological Applications Of P53-protected Noble Metal Nanoclusters

Over the years,the tumor suppressor protein p53 has been a well-known biomarker in biology and medicine because it was detected as a biological organism in cell lines where DNA damage was detected in tumor cells such as cell senescence,apoptosis,and metabolism in new cities.Superstar in the field of research.Its core domain(amino acid residues 94-292)plays an important role in specific DNA,and the 9 tyrosines and 9 cysteine residues involved provide reducing agents and ligands.The Au-S bond is further formed by a reduction reaction with gold to obtain a protein as a ligand-protected metal nanocluster such as BSA-AuNCs.Gold nanoclusters are considered to be one of the most popular nanomaterials.Compared with gold nanoclusters synthesized and protected by small molecules such as glutathione,dihydrolipoic acid and other ligands,biomacromolecules are used as ligands to protect gold.Nanoclusters have attracted much attention due to their low toxicity,large Stokes shift,good biocompatibility and potential biological functions,such as BSA,trypsin,pepsin,lysozyme and so on.p53-AuNCs are AuNCs that we designed and synthesized to protect against the cancer protein p53 ligand.This method has one-step reaction,simple and environment-friendly features.The synthesized p53-AuNCs emit red fluorescence(676 nm),smaller particle size(< 2 nm),stable to high salt and pH,and can be used as fluorescent probes to select and specifically detect anticancer drug small molecular drugs myricitone This can not only improve the complexities of HPLC detection of flavonoids and the costly use of equipment,but also provide new ideas for cancer drug treatment.Protein-protected gold nanoclusters are limited in their application due to their low quantum yield,while the doping of other metals(such as Ag)during the synthesis of metal nanoclusters can increase the optical properties of the synthesized metal nanoclusters due to the bimetal synergistic effect.the goal of.We also designed and synthesized AuAgNCs@p53 using the protein p53 as a ligand.This alloy protein gold-silver nanocluster has greatly improved synthesis time and fluorescence quantum yield compared to single metal nanoclusters.Further bio-imaging studies revealed that the synthesized alloy nanoclusters can enter and image in the nucleus,which provides a good imaging probe for further biological research.Furthermore,AuAgNCs@p53 retains the biological function of its ligand protein p53,which can inhibit the growth of cancer cells and alter the growth activity of cancer cells without affecting normal cells.