Bio-activity And Toxicity Analyses Of Bostrycin Derived From Endophytic Fungi HCH285

In this study,an endophytic fungus HCH285 was isolated and purified from the rhizome of Artemisia argyi in the laboratory.The HCH285 strain was identified,and its metabolites were separated and identified,and their toxicity and activity were analyzed.Furthermore,the endophytic metabolites were analyzed for the toxicity.The active compounds were isolated for the activity analysis as follows:1 Identification of HCH285 strain.HCH285 Endophytic fungi have been traditionally studied and described based on both morphological characteristics and molecular methods.In the present study,the results show that the colonies were rounded,with white fleece and fluffy growth in the early growth stage.The mycelia gradually became red in the late stage,and the red metabolism was observed on the back of the colonies.The mycelium was produced segmented hyphae and sporeless round conidia.Also,internal transcribed spacer?ITS?sequence was determined by using molecular techniques to find whether this strain belonged to Nigrospora oryzae.2 Compound preparation method for the separation of HCH285 endophyte metabolic pigment.Different chemical reagents were used to extract the metabolites,so ethyl acetate was selected as the best extraction reagent.The compounds were separated in semi-preparative liquid phase,and the HPLC method was optimized for the separation of monomer substances.The separation purity and method using 400MR structural analysis for bostrycin:the column temperature was 30?;injection volume:700?L;flow rate:3 mL/min;mobile phase:methanol:0.3%formic acid=50:50.3 Bostrycin bioassays.Antioxidant,antibacterial and the anticancer analysis of the bostrycin showed significant activity and provedas a good antioxidant,antibacterial and the anticancer compound.The order of the resistance to the three free radicals were O₂^–>DPPH^–>OH^–.CCK-8 in vitro showed that at the median lethal concentration U251and B10F16 cancer cells were about 4.30?M and 6.05?M.4 HCH285 Pigments and Toxicity Analysis of bostrycin.C.elegansalong withfood grade Monascus red was applied as a control treatment to assess the various characteristics of C.elegans.Results show that the acute and chronic toxicity of pigments produced from HCH285 strains and C.elegans were exhibited no significant effects on feeding ability,life history traits,and egg hatching rate.While food-grade monascus red at500?g/mL showed a consistent effect.Results also show that the toxicity analysis of bostrycin on C.elegansat the rate of 10?g/mL are not toxic for C.elegans.