| Cells are the basic building blocks of life.With the continuous advancement of science and technology,studying the structure,function,and behavior of cells is still an important issue that scientists continuously explore.Most of the traditional studies focused on the whole cell population,and the average level of each cell in the cell population was used as the research basis,ignoring the differences between cells and cells.This differences caused by the cells themselves and the microenvironment they are in is important to fully understand the cell-specificity and complexity of the tissue microenvironment under physiological conditions.Therefore,research on cells at single cell level is an important development direction in current cell research work.As an important part of life activities,trace elements can affect all aspects of life activities and are also one of the research objects of single cell analysis.Inductively Coupled Plasma Mass Spectrometry(ICP-MS)is one of the most important means of elemental analysis and has excellent elemental detection capabilities.It is able to detect the content of the cell's own elements at the single cell level,while also being able to analyze the level of exogenous elements(eg drug absorption,elemental markers,etc.)on a single cell.Research on single-cell analysis using ICP-MS has made considerable progress in recent years and is expected to play an important role in the fields of chemistry and biology.The purpose of this dissertation is to investigate the effect of various conditions on the results of single cell analysis during single cell analysis;The single-cell ICP-MS(SC-ICP-MS)method was applied to yeast and porcine sperm cells,respectively,and the element content levels of the cells were examined.At the same time,the adsorption of exogenous elements of the two cells was examined.The research in this article mainly includes the following:(1)Using yeast cells as the research object,a single-cell elemental analysis method was established to achieve quantitative analysis of Mg,K,Mn,Cu and Zn at the level of a single yeast cell.The single-cell signal difference,signal-to-background ratio,detection limit,and cell counting ability with a dwell time of 0.1-10 ms were examined.Single cell signals were quantified using standard solutions and a quantification method was established under short dwell time condition.The results showed that the signal duration of a single yeast cell was approximately 0.8-1 ms;the quantitative results obtained by the SC-ICP-MS method were consistent with the results of the cell digestion assay;using a lower dwell time could effectively improve the signal-to-background ratio,and at the same time lower detection limit.(2)Based on yeast ICP-MS single cell analysis,this method was applied to porcine sperm cell analysis.The cell integrity of the porcine sperm cells after atomization,the difference of the elemental signals before and after cell disruption,and the intake of the tumor drug cisplatin were investigated.The experimental results showed that the cells remained intact cell structure after atomization by the atomizer.Before and after the cells were broken,the changes in signal of single cells of different elements showed different trends.At the same time,the intake of antitumor drug cisplatin by sperm cells was investigated at the single cell level.The results showed that the Pt content in single porcine sperm cell was positively correlated with the incubation concentration and time of cisplatin,at the same time,the Pt content on the single cell level did not reach saturation with the increase of incubation concentration and time,reflecting the characteristics of cisplatin through free diffusion into the cell.. |
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