An Enhancer Screening And Organogenesis Analyses Of ANGUSTIFOLIA In Arabidopsis Thaliana

The organ shape and size are normally determined by the number,size and arrangement of cells in plant.Arabidopsis petal plays a model organ for its simple structure and difficulty of being influenced by external condition.ANGUSTIFOLIA in Arabidopsis is an animal homolog that encodes C-terminal binding protein,and plays role in regulating microtubules and controlling the anisotropic development of cotyledon pavement cell.This study has screened a dramatic mutant by the chemical induction of an-2 with Ethyl methyl sulfonate,an-2 eoa(EOA,Enhancer of ANGUSTIFOLIA),which significantly inhibited the isotropic development of petal,and has gotten the single point mutant,eoa,by crossing separation method.The analytical results showed that the width of petal and cotyledon of an-2 were strikingly narrower compared to the wild type,however,the length of those had no significant changes.Simultaneously,the petal and cotyledon of an-2 eoa displayed longer and narrower phenotypes.Furthermore,the abaxial cell length and size of an-2 petal became larger.eoa mutant showed longer and narrower cell,an-2 eoa shared similar cell phenotypes with eoa,additively,the cell size was no significant change compared to an-2.On other point,the adaxial cell of an-2 showed increased length and reduced number.The cell of both eoa and an-2 eoa showed narrower width and reduced size.Deeply study,the conical cell of an-2 and an-2 eoa displayed swollen tip,and the microtubules in conical cell showed disordering orientation.Conclusively,this study found EOA is really an enhancer of AN according to the statistics analyses,this two proteins are involved in the process of growth and development of Arabidopsis,regulates the microtubules arrangement to control cell anisotropy to finally influence organ morphogenesis.