Regulation Of Development Of Mouse Bergmann Glia Cells By Histone Methyltransferase SETDB1

The cerebellum is an important organ that controls movement coordination and motor learning.In recent years,studies have found that the cerebellum is also involved in complex learning and memory cognitive and behavioral manifestations,and is closely related to diseases such as ataxia and autism.Epigenetic modification such as H3K9me3 plays an important role in development,but how it regulates the development of cerebellar cells remains unclear.This paper is to investigate the function and molecular mechanisim of H3K9me3 modification in Bergmann glia of cerebellum by constructing different conditional knockout mouse models.The findings will broaden the understanding of the regulation of cerebellar development,providing a theoretical basis for cerebellar related diseases.We use mGFAP-cre conditional knockout mice,which specificly knockout Setdb1 in neural stem cells,so that we can study the effect of Setdb1 deficiency in cerebellum during mouse development.Our results suggest that the cerebellum development of the knockout mice is severely impaired,and the ability to exercise and balance are declining.The migration of cerebellar granule neurons was abnormal,and it was determined that the abnormal migration was caused by the damage of the Bergmann fibers.So far,we initially found the cellular mechanism that caused the abnormal development of the cerebellum.Afterwards,we explored the molecular mechanisms that caused cerebellar development defects through techniques such as RNA-seq to further elucidate the function of H3K9me3 in the development of cerebellum.