Study On The Secondary Metabolites From Cinnamourmus Endophytic Fungi Strain Y-74 And Chestnut Blight Fungus 155

With various species and high metabolic-activity,plant fungi can also produce and accumulate a number of active secondary metabolites with unique structures and novel skeleton through the metabolic process.Plant fungus play a very important role in drug discovery and development in areas such as natural pigments.The secondary metabolites of endophytic fungi generated from parts of the medicinal plants exhibit much similar or even the same activity with their host plants,which can solve the shortage problem of medicinal plant resources.Meanwhile,as one of the important sources of natural pigments,the plant fungal pigment can not only overcome the insecurity of synthetic pigments,but also solve the raw material shortage by mass production via the fermentation.Given the vital functions of plant fungus on the research and development of drugs and natural pigments,etc.,the research and development of their secondary metabolites is a new path to study the natural products,which is environmental benign and good for the ecology protection and sustainable development.The paper was aimed at Cinnamourmus Endophytic fungi strain Y-74 and stain chestnut blight fungus 155.Firstly,Cinnamourmus Endophytic fungi strain Y-74 is an Endophytic Bacteria with antibacterial activity which was screened from Nanning Liang Feng River National Forest Park,and whose phenotype and morphology were observed after the colony culture.Then its secondary metabolites after liquid fermentation were purified,analyzed and identified.The result turned out that the frontage of Y-74strains presented green and villous with the neat edge,while the reverse side was bluish yellow and easy to produce spores.The mycelia colony on the edge was silvery white and opaque with dry surface,while the mycelium was slender with large sporulation quantity which can produce a large number of globular conidia.After fermentation culture,its fermentation liquid was.isolated and purified by silica column chromatography method,and 11 components were abained,among which three components(2),(4),(5)exhibited certain antibacterial activities through bacteriostatic experiment.After the identification on GC-LC,the component(2)was found to contain two substances.Furthermore,one substances(2)-2 was purified and obtained,but the antibacterial test comfirmed that this substance lacked the antimicrobial activity.NMR spectra was applied to identify the substance as citrinin A(penicitrinone A)whose formula is C23H24O5.As the literature reported,citrinin A is a drug with strong anti-tumor activity.At the same time,the corresponding research was carried out to the stain chestnut blight fungus 155.First of all,the growth and pigment production of strain 155 were investigated.It was found that after cultured for 15 d,The cumulative amount of pigment got to the maximum,and such pigment was fat soluble,and easy to solve into the organic solvents like methanol,ethanol,ethyl acetate,acetone,et al.,and esp.in chloroform and dichloroethane but insoluble in water.Secondly,the methods on the extraction of pigment was discussed in detail and the result found that solvent extraction was suitable for the pigment extraction using methanol as the extraction agent and a water bath at 60? for extraction 1h to produce a pigment solution with the maximum absorption wavelength of 458 nm.When detected the stability of the pigment,it possessed a good heat resistance and a good light stability under UV irradiation,but the outside sunlight presented a certain influence on its stability.The pHwas stable in the range of 4-9.Besides,it also emerged a relatively good stability to most metal ions,oxidizing agent,reducing agent,food additives and preservatives.Solid state fermentation of stain 155 pigment has been elementarily separated and purified by using silica gel column chromatography,and then two components were obtained from the pigment,whose purities were analyzed by HPLC.The molecular weight of the compound 155-1 is measured as 536.62,and 155-2 is 552.07 by MS.Morever,the structures were characterized by nuclear magnetic resonance spectra to confirm that the two substances are novel compounds.