The Anti-aging Effect Of A-ketoglutarate On Drosophila Melanogaster

Alpha-ketoglutarate(AKG)is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.As a precursor of glutamate and glutamine,AKG plays important roles in various aspects of organism metabolism.It is one of the ingredients of nutrition beverage on sports,organic intermediates,biochemical reagents and test kit reagent on liver function.It can reduce the body loss for postoperative patients and long-term patient.In the brain,it is as a precursor of tyrosine and glutamic acid.It is also a cyanide resistant.In addition,recent research has found AKG can prolong the life of nematodes in the culture medium.However,about if the AKG can prolong the life of flies and their corresponding anti-aging mechanism is unclear.In the present study,we mainly explored the anti-aging effect of AKG on Drosophila melanogaster.The results demonstrated AKG can significantly extend the lifespan of female's fruit fly,but reproductive performance was reduced.In other physical indicators,we found AKG significantly improved the ability of fly and anti-heat shock and no adverse impacts were found.On the aspect of metabolism mechanisms,we found AKG reduced the ATP/ADP ratio and increased the level of autophagy.This indicated AKG may active the signing pathway of AMPK and inhibit ATP synthase and mTOR.By further studied these related pathway,we confirmed AKG extend the lifespan of female 's fruit fly by active the signing pathway of AMPK and inhibit mTOR.ATP synthase may also be inhibited but it need further studies to prove.Furthermore,we also detected some other pathways and explored their roles in the process of metabolism.