Study On The Growth Promoting Effect Of Bacillus Velezensis

In recent years,in order to meet the needs of plant growth and increase the income of agricultural and forestry products,the use of fertilizer has been increasing.In addition,the unreasonable use of fertilizer by farmers has brought a lot of trouble to the ecological environment.But bacillus is the natural and no destroy to the environment.It also has many fine characteristics.,such as:breeding speed,Sporing to adapted to the adverse environments,and it can inhibit pathogens by antagonizing or producing metabolites.Because of these advantages,Bacillus is provided with very important economic value.In this paper,Bacillus velezensis was used as the research object,and the organic and organic extracts of the organic reagents were obtained by continuous extraction of petroleum ether,chloroform and ethyl acetate.After the rotary evaporation,the crude extract was obtained.The effects of different dry matter concentrations on seed germination and seedling growth of maize were studied by diluting 10 kinds of dry matter with 10 times,100 times and 500 times respectively.The results showed that the crude extract of chloroform could promote the early germination of maize to different extent,and the seeds had high germination index.The crude extracts of petroleum ether,ethyl acetate,chloroform and water phase have the best effect on the root length of corns.In conclusion,the effect of chloroform extract is the best.The chloroform extract was diluted 10 times,100 times,300 times and 500 times,respectively,which was used to soak and irrigate the corns and lima beans.Experimental results show thatthe chloroform extract could effectively increase the germination potential and germination rate of peas.The chloroform extract alsocould significantly increase theshoot length of the corns and lima beans.The corns,peas and lima beans after soaking chloroform extracts,the root dry weight,fresh weight and leaf dry weight and fresh weight of which were significantly increased.Through irrigation,chloroform extracts can significantly improve the corn Germination potential and germination index and germination index of lima beans.There was no significant change in the germination of peas compared with the contrast.The root length,bud length,root dry weight and fresh weight and leaf dry weight and fresh weight of corn,peas and lima bean were improved in different degree.According to the results,the seed soaking and root irrigation could achieve a certain growth promoting effect,and the effect was better when the crude extract was diluted 300 times.The crude chloroform extracts,which were diluted 10 times,100 times,300 times and 500 times respectively,were soaked with Podocarpus macrophyllus and Albizzia julibrissin.The results showed that chloroform extracts can improve the germination index,root length and shoot length of Podocarpus macrophyllus and Albizzia julibrissin.It also can increase the SOD activity,POD activity and chlorophyll content in leaves.It can reduce the concentration of MDA in leaves.So that the seeds of trees have a good start.In order to improve the stress resistance and increase the yield of the plant,it laid a good foundation.