The Study On The Secondary Metabolites From A Strain Of Marine-derived Fungus And Their Activity On Vibrio Anguillarum

Ecological environment has great impact on fungi's secondary metabolites.Because of the special living space,marine-derived fungi's secondary metabolites are different from other fungi's secondary metabolites and a few peptides possess activity on Vibrio anguillarum.The thesis was devided into four chapters.In the first chapter,the structure of the secondary metabolites from marine-derived fungi and the research progress of their activity on Vibrio anguillarum were reviewed.In the second chapter,the experiment was guided by OSMAC strategy and screened a strain,and 10 compounds were isolated from the crude extract.In the third chapter,10 compounds' structures were elucidated by extensive sptectroscopic techniques.They are(-)-Cyclopenol?(-)-Cyclopenin?3-ethylidene-6-isopropyl-piperazine-2,5-dione?Cyclo (L-Pro-L-Leu)?xylariolide D?roquefortine C?Cyclo(D-Pro-L-Tyr)?Cyclo(Phe-Ala)?Cyclo(Val-Ala)?Cyclo(4-hydroxy-proline-isoleucine).In the last chapter,a method to evaluate the activity on Vibrio anguillarum was established,positive-drug enrofloxacin and 10 compounds' activity on Vibrio anguillarum were tested.The results showed that enrofloxacin's MIC is 16 ?g/mL,and compound 1-10 showed no activity on Vibrio anguillarum.