Antagonism Tributyltin Toxicity Effect And Its Mechanism Research Of Fucoxanthin,Fucoxanthol And Antioxidant Peptides

Tributyltin(TBT)is a wide range influential persistent organic compound.In-depth studies have been carried out on the toxic effects of TBT on organisms and the mechanism of action and the pollution control issues.However,how to alleviate the toxic effects of TBT and reduce the harmful effects of TBT on the health of the body is still lack of a broad and deep research.HepG2 cell lines and C57BL/6 mice were used as models in this study,the antagonistic effects on TBT-induced toxicity and its possible mechanism of Fucoxanthin(Fx),Fucoxanthol(FxOH)and Antioxidant Peptides(AP)were studied in vitro and in vivo.The paper obtains the following results:(1)Fx,FxOH and AP have a significant antagonistic effect on TBT-induced decrease in cell viability.Fx,FxOH can effectively remove TBT-induced ROS.They had significant antagonistic effect on the increase of MDA content in HepG2 cells.(2)Fx,FxOH and AP have a significant inhibitory effect on apoptosis induced by TBT.Fx,FxOH and AP play an inhibitory role on TBT-induced cell apoptosis by effectively increasing the expression of Bcl-2 protein and decreasing the expression of Bax protein,indicating that these three marine natural products inhibit TBT-induced apoptosis via the regulation of Bcl-2 and Bax.(3)The Sperm tail deformity rate of TBT-administered mice was significantly higher than the control group,while AP could effectively inhibit the Sperm tails deformity by TBT.