Human-specific MiR-941 Function In Neural Progenitor Cells

Human-specific miRNA miR-941 is highly expressed in human brain tissue and may play an important role in human brain evolution.In order to explore its possible function in human brain development,miR-941 was stably overexpressed in human neural progenitor cells(ReNcell CX),and the phenotype of miR-941 overexpression was identified in terms of cell morphology,proliferation,migration,senescence and apoptosis.The results showed that overexpression of miR-941 altered the human neural progenitor cells morphological and inhibited the proliferation.Transwell chamb assay found that the number of cell migration increased.The results of apoptosis and senescence showed that miR-941 may promote apoptosis and senescence of human neural progenitor cells.At the cellular level,immunofluorescence staining revealed that microtubule structure of overexpression cells is disorder,the distribution of perinuclear enrichment area changed.While the number of stress fibers in the microfilaments decreased and the number of lamellipodia increased.Indicating that miR-941 overexpression caused abnormalities in the cytoskeleton of human neural progenitor cells.The expression of the microtubule-structured protein gene in the neuronal cell is abnormal and further supports its influence on the cytoskeleton.At the same time,The expression levels of the sternness marker genes NES and Sox2 were detected and those genes show decreased in the overexpressed miR-941 neuronal progenitor cells.The phenotypic differences suggest that function of miR-941 in neural involves in proliferation,migration,and abnormal assembly of microtubules,it has provided us a better understanding of the role of miR-941 in the development of the human nervous system.