Isolation And Characterization Of Cellulose-degrad Ing Related Bacteria From Soil

Cellulose is the most abundant renewable resource,which is used for the production of clean energy and many important chemical products material,thus the degradation and utilization of cellulose has been the focus of all countries' scientists.Some bacteria can degrade cellulose without secreting extra-cellular cellulose enzymes or containing cellulosome,thus its degradation mechanism has been a mystery.A strain was isolated from soil and conserved in our lab.Physiological and biochemical characteristics of this strain were studied.Together with the 16S rDNA identification and cell-wall fatty acid identification,the taxonomy of this strain was primarily identified.Also,the growth properties of the strain were partially investigated.In this work,the process the strain degraded the filter paper fibre was studied by means of scan electronmicroscopy.The filter paper cellulose degraded rule and the morphology change on different phase were analysed.The result showed that in the process of degrading fibre,the strain adhere tightly to the fibre through its bacilliform cell.The bacilliform cell penetrate into interior of the fibre to degrade the cellulose strongly and produced a mass of sticky polysaccharides.