The Study On The Protection Of Glycine To Chorispora Bungeana Under Different Kinds Of Stress

Glycine is one of the most important amino acids in life activities, plays an irreplaceable role in organism metabolism. In this research, we used Chorispora bungeana as experimental material, studying with the Phospholipase D which we had already known the sequences in Chorispora bungeana, to explore the protective effect of glycine to Chorispora bungeana under different kinds of stress. In our experiment,we measured the relative electrolytic leakage, the malondialdehyde content, the proline content, soluble sugar content, the catalase activity, the superoxide dismutase activity, the peroxidase activity, the PLD mRNA relative expression, the PLD protein expression, the activity of PLD in Chorispora bungeana, to explore the protection of glycine to Chorispora bungeana. The results are as follow:(1) First part we research the affection of Glaucocalyxin A to Chorispora bungeana. Glaucocalyxin A is a kind of diterpenoid compound which extracted from Isodon racemosa(Hemsl) Hara. Using 50,100,200?M of Glaucocalyxin A as thetreatment for 72h: As the indicators of membrane damage: electrolyte leakage and malondialdehyde contents, as the indicators of osmotic adjustment substances: the content of proline and soluble sugar, and the contents of three kinds of antioxidant enzymes shared similar trends that increased significantly and peaked at hour 24 and then decreased when C bungeana buds was treated by GLA. The activities of PLD exhibited an upregulation behavior, with the highest levels at 24 h, respectively.RT-PCR analyses suggested that PLD activity partially corresponded to the C bungeana PLD gene transcript level. The expression of PLD protein was affected by GLA and it reached the peak at 24 h. These results demonstrated that the specific mechanism of C bungeana response to GLA was linked to PLD, But the effect was gradually decreased with time.(2) Second part we explored the protective effect of glycine to Chorispora bungeana. We treated the Chorispora bungeana with cold stress, salt stress, drought stress and allelopathic potential material stress for 72 h. The electrolyte leakage and malondialdehyde contents, the content of proline and soluble sugars and the contents of three kinds of antioxidant enzymes also shared similar trends that showed significant upward changes in salt stress with glycine and salt stress, however, the restof three treatment showed no obvious changes with the control. The activities of PLD exhibited an upregulation behavior, with the highest levels at 48 h in salt stress with glycine and salt stress. RT-PCR analyses suggested that PLD activity corresponded to the C bungeana PLD gene transcript level in salt stress with glycine and salt stress.However, the rest of three treatment showed no obvious changes with the control. We suggested that glycine could protect the Chorispora bungeana under cold stress,drought stress and allelopathic potential material stress. But the damage caused by salt stress was not protectable by glycine in this experiment.