Enzymatic Properties Of Polyvinyl Alcohol Degrading Bacterium And Analysis Of Degrading Genes

Polyvinyl alcohol?PVA?,as a kind of high performance polymer materials,was used in many fields of production and every aspect of science research.Meanwhile the overuse of PVA resulted in environmental pollution.The strain HK1 was isolated from environment,which could degradation polyvinyl alcohol.The PVA-degrading enzyme producing conditions and enzymatic properties of strain HK1 were studied.Then the gene of PVA-degrading enzyme of the strain HK1 was preliminarily analyzed.The main result was followed:?1?The strain HK1 was characterized as Bacillus amyloliquefaciens by testing its physiology,biochemistry,and molecular identification.?2?It was showed that the extracellular enzymes of the strain HK1 was dominant by observing the colorless transparent circle and testing enzyme activity distribution.?3?Optimization of culture conditions was carried out through mono-factor and orthogonal experiments.The optimum fermentation conditions were as follows: volume ofmedium was 50 mL,inoculability quantity was 6%,culture temperature was 30?,the pH of culture-solution was 7.0,and the concentration of PVA,FeSO₄,MgSO₄,CaCl₂,NH₄NO₃,and yeast extract were respectively 2.5 g/L,0.05 g/L,0.7 g/L,0.05 g/L,0.6 g/L and 2.5 g/L.Under this fermentation condition,the production of degrading enzyme of strain HK1 was 125% higher than when it was cultured in former fermentation condition.?4?The optimal degradation conditions of PVA-degrading enzyme were: 1.5 g/L PVA,30? and pH 7.0.?5?Some genes which could be associated with PVA degradation were preliminarily studied through analyzing genome information of the strain HK1.