Complete Mitochondrial Genomes And Phylogeny Study Of Lamprotula(Unionidae)in China

Lamprotula Simpson 1900 only distribute in Asia.Most Lamprotula species are China,and only a few of them are found in Japan,North Korea and Indochina.China is rich in the resource of Lamprotula.However,since the mussel's habitats are increasingly destructe endemic to China in recent years,the wild populations of Lamprotula are now suffering serious depression.In this study,to determine the complete sequence of mitochondrial genome,we carried out the LA-PCR reaction and primer-walking,and conducted the mitochondrial genomics and phylogenetic analysis.While the classification of them have been controversia.Following are the main results:1.This study firstly determined the complete F type mitochondrial genomes of Lamprotula tientsinensis and Lamprotula rochechouartii,with the genomes length 15965bp and 16104bp.2.The 7 mitochondrial genome benlong to 6 specise of Lamprotula possess 4 kinds of arrangement model,and the F type of Lamprotula tientsinensis,Lamprotula tortuosa,Lamprotula gottschei,Lamprotula coreana with same arrangement model.Lamprotula leai and the species of Gonideinae possess same mitochondrial genome arrangement model.The F type mitochondrial genome of Lamprotula rochechouartii and the species of Margaritiferidae have the same arrangement model.All the M type mitochondrial genomes which have been released share only one arrangement model.3.Through phylogenetic analysis,The Lamprotula is polyphyly,and 3 different evolution clades belong to Margaritiferidae,Gonideinae and Unioninae.4.We carried out the analyses of COX1,16SrRNA and ND1,and found that several individuals with significant differences in morphorlogy are from the same species.These results suggest that there could be great morphological variations among different individuals from the same species.