Study On Reproductive Defects And Molecular Genetics Of Arabidopsis Kd361 Mutant

Male and female gametophyte development,double fertilization and embryo development are essential steps in the process of sexual reproduction from angiosperms.In an Arabidopsis thaliana mutational bank,we derive a mutant kd361 which more deviates a constant from Mendel's inheritance laws.Its seeds shrink into dense granules accounted for 32.78%in a silique and transfer rates of male and female gametes are 39.91%and 48.81.17.26%unfused polar-nuclei in final sac morphogenetic stages,regular Pollens germination but a flawed tubes growth adaptability after pollination,make kd361 36.13%embryos to Call off fertilization or zygotes cleavage in early globular embryo stage.TAIL-PCR and molecular genetic anaysis show that Ds maps between At4g00380 and At4g00730 gene article IV chromosome.At the same time,the Ds transposon causes continuous linear 39 genes lost.They are lethal homozygous embryo mutants that the missing At4g00620 gene's allelic mutants sail₇20_g12 and salk₁41636aborte 25.55%in the lost section.It is able to fully recover allelic deficient phenotype,and reduce to around 20%seeds abortion of kd361,but not a homozygous Ds plants from our genetic complementary molecular result.Afurther research that kd361 transgenic homozygous plants's female gamete transfer rate is up more than 80%,but the male gamete do not change significantly shows the gene regulated kd361 defects,but controlled by more.At4g00620 gene,a amino acid dehydrogenase family protein involved in the synthesis and metabolism of folate,plays a key role in plant's embryonic development that a disruption from folic acid synthesis and metabolic will cause future generations about 1/4 embryos abortion conducted by our studies for the first time.