Application Of GO/BaHoF 5 Nanocomposites In Tumor Multimodal Imaging And Photothermotropic Therapy

Graphene is one of the most fantastic 2D carborn material newly found these years.Among the derivatives of graphene,graphene oxide is well known for rich functional groups on surface and high water solubility.Thus,a large number of methods,especially in suit growth of inorganic nanoparticles,have been adopted to prepare multifunctional graphene oxide nanomaterials.In this way,graphene oxide was endowed with porperties from nanoparticles such as CT,MRI and photoacoustic imaging.Furthermore,genes and drugs are able to get loaded and transported to tumor regions by graphene oxide via non-covalently force.In this dissert,we prepared graphene oxide nanosheets with BaHo F₅ nanoparticles grown on the surface.NVP-AUY922,a novel heat shock protein inhibitor,was then loaded on it.We studied the in vitro uptake and distribution of GO/BaHoF₅/PEG nanosheets.The Hsp90inhibited phototheraml therapy of cancer cells was also well-performed.Moreover the CT/MRI dual-modal imaging ability of GO/BaHo F₅/PEG nanosheets was researched and in vivo tumor CT imaging was proved to be effective.This dissert was divided into four parts.In the first chapter,we talked about the development and classification methods of graphene and its derivatives.Preparation and modifications were also reviewed.At the end of this chapter we bring up the research topic of this dissert.Next section,we obtained nano-sized graphene oxide by ultrasound stripping of graphite and prepared GO/BaHoF₅/PEG nanosheets by one step solvothermal methods.The GO we made were proved to be a single sheet in thickness by AFM and the remaining sulfate in GO was cleared by dialyzing in a two-month period.The BaHoF₅/PEG nanoparticles was synthesized and various ratios of GO,Ho,Ba and F were tested to acquired GO/BaHoF₅/PEG nanosheets in a uniform size and nanoparticles density.Moreover,the loading capacity of BaHoF₅ was also determined by thermal gravimetric analysis.The stability of GO/BaHoF₅/PEG nanosheets in physiological solution was also verified.In part three,we characterized the MRI/CT imaging abilities and heat shock protein inhibitor loading enhanced photothermal therapy of GO/BaHoF₅/PEG nanosheets.First,the photothermal,CT and MR imaging properties of GO/BaHoF₅/PEG solutions were investigated.Then confocal laser scanning microscope and flow cytometry were used to observe the performance and distribution of FITC modified GO/BaHoF₅/PEG nanosheets in cancer cells.NVP-AUY922,a new heat shock protein 90 inhibitor,was then loaded on GO/BaHoF₅/PEG nanosheets.Sensitized photothermal treatment was performed in cancer cells and considerable Hsp90 reduction was observed by western blot.Furthermore,the in vitro/vivo biosafety experiments were carried out and the material had little cytotoxicity,almost no hemolysis,and no effect on both body weight.Blood indexes and pathological sections were also proved to be normal.Notably,the MRI signals of GO/BaHo F₅/PEG nanosheets were remarkable and in vivo tumor CT iamgings showed srtong contrast.It is rational to say that GO/BaHoF₅/PEG nanomaterial is a promising candidate for future tumor imaging and photothermal therapy.At last,I summarized my research over graduate period and discussed the advantages and disadvantages of this work.The future development of this filed was also involved.