| Objectives:The model of rabbit in situ liver cancer was established,with incomplete radiofrequency ablation applied to carcinoma lesions,to simulate the clinical treatment for one large hepatocellular carcinoma or multiple hepatocellular carcinoma lesions by radiofrequency ablation before being treated with andrographolide(XIYANPING)to inhibit the tissue inflammation of after radiofrequency ablation.Comprehending its inhibition of the secondary inflammatory response resulted from tissue damage after the radiofrequency ablation by detecting the level of serum inflammatory cytokines,detecting the variation of proliferation and invasion of residual tumor cells after intervention by immunohistochemistry,investigating,compared with the carcinoma cells without intervening medicines,whether the andrographolide can inhibit the invasion and metastasis of residual hepatocellular carcinoma cells after radiofrequency ablation by way of anti-inflammatory effect so as to perform an anti-tumor effect,all of which provides a theoretical basis for the combined application of radiofrequency ablation with anti-inflammatory andrographolide to decrease the recurrence and progression of hepatocellular carcinoma.Methods:New Zealand Rabbit VX2 animal model was established.Eighteen model rabbits after radiofrequency ablation were randomly divided into three groups,6 rabbits in each.In the following 7 days,the rabbits were intraperitoneally injected with medication daily.Andrographolide(XIYANPING)with the dose at 10mg/kg/d,which was refered to the children dosage,was applied to the XIYANPING group,Aspirin was applied with the dose at 25mg/kg/d to the aspirin group,while the control group:saline at 10mg/kg/d.The serum inflammatory factors IL-6 and HS-CRP of the 1st,3rd and 7th day after ablation were detected by ELISA.Four weeks after ablation,all the animals were executed to collect specimens.The expression of VEGF in each group's residual ablation lesions was detected by immunohistochemistry,and meanwhile,the number of distant metastasis from residual lesions was counted after collecting the lung and kidney tissues.Results:The levels of IL-6 in XIYANPING and aspirin groups all show descending tendencies in contrast to that in the control group on the 1st,3rd and 7th day after radiofrequency ablation,whereas the tendencies were not statistically different(P>0.05).The levels of HS-CRP in XIYANPING and aspirin groups were statisticallylower than that of the control group on 3rd day after radiofrequency ablation(P<0.05).Four weeks after ablation,the expression of VEGF of residual carcinoma lesions in XIYANPING or aspirin group descended comparing that in the control group,and the differences were statistically significant(P<0.05).No metastatic lesion was founded in the kidneys in all groups,and yet there were metastases present to some extent in all lungs.Compared with the control group,the numbers of metastatic lesions on the lung surface in XIYANPING and aspirin group were less,which were statistically different(P<0.05).Conclusions:Andrographolide can significantly inhibits the secondary inflammatory response resulted from tissue damage after the radiofrequency ablation of hepatocellular carcinoma,and the inhibiting effect of andrographolide to the inflammatory response after radiofrequency ablation of hepatocellular carcinoma was no less than that of aspirin.Inhibition of inflammatory response after radiofrequency ablation of hepatocellular carcinoma by andrographolide,to some extent,can delay the progression of residual tumor.The andrographolide may play an anti-tumor role by way of anti-inflammatory effect that delays the invasion and metastasis of residual hepatocellular carcinoma cells.The combined application of radiofrequency ablation and anti-inflammatory medication to hepatocellular carcinoma could possibly be a new therapeutic approach to improving the prognosis 'of liver cancer and prolonging the survival time of patients. |
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