Study On The Quality Standards Of Black Berry

Objective The aim of this study was to establish a set of quality evaluation criteria for Lycium ruthenicum Murr.by taking Gouqizi as reference and combining with the study of its liver and intestinal metabolism.Based on the control of the content of major components,the control of monomer chemical components,the overall fingerprint of medicinal herbs and the fingerprint of its diagnostic component,this study provided a multiple reference for the overall quality control of Lycium ruthenicum Murr.;At the same time,the study on the metabolism of Lycium ruthenicum Murr.can further advance the active ingredient of Lycium ruthenicum Murr..Methods 1.Analyze the chemical constituents in the extracts of Lycium ruthenicum Murr.by LC-MS,this would provide clue for the selection of the reference materials in the experiment of identification and assay.2.Using the rat in vivo closed intestinal ring method,combined with LC-MS technique to study the metabolism information of the water extracts of Lycium ruthenicum Murr.,to lock the chemical constituents into the blood system by the original or metabolic form,as the selection basis of the index components in the content detection.3.Using HPLC technique,the whole fingerprint and diagnostic component fingerprint of Lycium ruthenicum Murr.were established,and the composition of Lycium ruthenicum Murr.was evaluated.4.To establish a method for determining the content of major components(polysaccharides,flavonoids,polyphenols and procyanidins)and index components in Lycium ruthenicum Murr.Results 1.With the help of the literature report,mass spectrogram shows there are 49 components were found in Lycium ruthenicum Murr.which contains alkaloids(betaine,amide alkaloids,etc.),anthocyanins category(mainly petunia anthocyanin),tannins(mainly coffee tanning),amphetamine(propionic acid,coumarin derivatives),flavonoids(rutin,naringenin,etc.)and other chemical constituents.2.In combination with the literature report,using the LC-MS method to identity the newborn components in the plasma of the Lycium ruthenicum Murr.metabolism animal experiment,it is speculated that the chemical constituents of the blood are:p-coumarin acid,N-acetyl-tryptophan,ferulic acid naringenin,etc.the chemical constituents of the blood are:caffeetannins,flavonoids and so on.3.The fingerprint of Lycium ruthenicum Murr.and the proanthocyanin oligosaccharide were established,and the similarity of the samples of Lycium ruthenicum Murr.was evaluated by using the software of "The similarity evaluation software of Chinese Traditional medicine chromatogram fingerprint 2012 edition".4.Based on the mass spectral analysis of Lycium ruthenicum Murr.,and with the help of refrences,the control materials and methods of determining the content of polysaccharide,flavone,polyphenol and procyanidins were established appropriatly.5.With the standard of Gouqizi in Chinese pharmacopoeia(2015 edition)as a reference,this study also selected betaine as one of the index components of quality control of Lycium ruthenicum Murr.,and established a set of HPLC-UV method for determination of betaine content in Lycium ruthenicum Murr.samples.6.Combined with the experimental results of rat liver and intestinal metabolism,the chemical constituents,which can be absorbed into blood system by original or metabolic form,were selected as optional content control index.Finally,chlorogenic acid,p-coumarin acid and rutin were selected as the content control index component,since the content of each among them were higer than 0.01%.And a corresponding HPLC-UV determination method was established.Conclusion and significance This study was based on the establishment of the fingerprint identification method of Lycium ruthenicum Murr.,the control method of major component and monomer component content,and the guiding principles of "integrity","multi-component control" and "detection method is related to active substance" in quality control of traditional Chinese medicine.The method can be used to characterize the chemical constituents of black fruit wolfberry,and can be applied to distinguish and appraise the origin and quality of Lycium ruthenicum Murr.