The Regulation Of Blood Exhaustion On Oxidative Stress Injury In Chronic Refractory Wounds In Rats

Objective:The rat model of chronic irreversible wounds with oxidative stress injury was established by superimposing epirubicin combined with skin defects,to further study the regulation effect of dragon's blood on oxidative stress in chronic refractory wounds.Methods:117 Wistar rats were randomly divided into four groups:blank group,simple lesion group,refractory model group,and blood group.There were 9 blank groups and 36 other groups.Except the blank group,the other three groups were randomly divided into four subgroups:3rd day group,7th day group,14th day group and 21st day group,9 in each group.Simple skin injury group were punched a diameter of 1.5cm hole on the back playing with a special hole punch,On this basis,Chronic refractory wound model group rats were injected epirubicin(2mg/ml)of 0.15ml in the wound edge skin.The dragon's blood group was administered to the Dragon Blood Ointment and the wound was evenly applied to the wound.The other two groups were evenly coated with vaseline,and the wound was covered with a disposable sterile dressing.In the simple skin lesion group,the refractory model group,and the Dragon's Blood group,the general condition and healing rate of wounds in each group were observed on the 3rd,7th,14th,and 21th days after modeling respectively,and the materials were obtained.The blank group was obtained only on the 3rd day..Paraffin sections were made and histological changes and macrophage counts were observed by HE staining.Im-munohistochemical assay for the expression of TLR4.Make wound tissue homogenate,Detect SOD activity and MDA level of wound tissue homogenate.Record the results and use spss18.0 for statistical analysis.Result:1.The healing rate in the refractory model group was significantly lower than that in the simple lesion group(P<0.01).2.Histological observation:Compared with the Simple skin injury group,the inflammatory cells in the refractory model group and the Dragon's blood group had a long infiltration time,fewer neo-microvessels,fewer collagens,and irregular alignment.Compared with the refractory model group,the time of infiltration of inflammatory cells in the dragon's blood group was short,new blood vessels appeared early,and the number was large,and collagen was numerous and neatly arranged.3.TLR4 expression:The TLR4 expression in the Simple skin injury group,the the refractory model group,and the Dragon's Blood group was significantly higher than that of the blank group in each observation period.During the whole observation period,the expression of TLR4 in the Simple skin injury group,the refractory model group and the Dragon's Blood group was significantly higher than that in the blank group(P<0.01).During the whole observation period,the expression of TLR4 in the refractory model group and the Dragon's Blood group group was significantly higher than that in the Simple skin injury group(P<0.01).Compared with the refractory model group,the dragon's blood group significantly increased on the 3rd and 7th days(P<0.01),and decreased significantly on the 14th and 21st days(P<0.01).4.SOD vitality and MDA levels:Compared with the blank group,on the 3rd,7th,14th,and 21th days of the refractory model group and the Dragon's blood group,the SOD activity was significantly decreased(P<0.01),and the MDA level was significantly increased(P<0.01);On the 3rd and 7th days,the SOD activity of the homogenate was significantly lower than that of the blank group(P<0.01),and the MDA level was significantly increased(P<0.01).On the 14th day,compared with the blank group,there was no significant difference in SOD activity(P>0.05),and the level of MDA was significantly different(P<0.05).On the 21st day,the SOD activity of the simple lesions group was not significant.There was no significant difference between MDA level and blank group(P>0.05).Compared with the simple group,the SOD activity in the blood-dried group and refractory model group decreased on the third day(P<0.05),and the MDA level increased significantly(P<0.01).On the 21st and 21st days,the level of MDA significantly decreased(P<0.01),and the level of MDA increased significantly(P<0.01).Compared with the refractory model group,on the third day,the SOD activity of the blood-exhausted group increased(P<0.05),and the level of MDA was significant.Decrease(P<0.01).On the 7th,14th and 21st days,the SOD activity in the blood-exhausted group was significantly increased(P<0.01),and the MDA level was significantly decreased(P<0.01).ConclusionDragon's blood can effectively promote the healing of oxidative damage chronic refractory wounds and improve the healing rate of wounds.The mechanism may be to inhibit the overexpression of TLR4,reduce the infiltration of inflammatory cells and reduce the inflammatory reaction time;reduce the level of MDA in wounds and increase the activity of antioxidant enzyme SOD to exert anti-oxidative stress injury.