Effect Of Yiqi Xiaozhen Tongluo Recipe On The Regulation Of JAK/STAT Pathway In Airway Remodeling In COPD Rats

The content of this paper is mainly composed of literature review and animal experiment.1.literature review.This article mainly discusses the etiology of chronic obstructive pulmonary disease from the perspective of modern medicine,the main pathogenesis and the main treatment methods.Review two combined chronic obstructive pulmonary disease and traditional medicine,this paper expounds the category of COPD in traditional medicine,etiology and pathogenesis,the treatment methods of ancient physicians and the inheritance of ancient experence,innovation of this subject.2,Experiments on animalsResearch purposeBy different doses of yiqixiaozhengtongluo formula play a role on the COPD rats model,to observe the airway in airway smooth muscle,fibroblast,the change of the epithelial cells and inflammatory cells,and response to chronic airway inflammation related IL-6,JAK1,STAT3,P-STAT3,JAK1mRNA STAT3mRNA SOCS3mRNA expression,the effect of which from the Angle of molecular biology to explore yiqixiaozhengtongluoformula can strengthen party for the mechanism of action of airway remodelling and concentration-response relationship,prove yiqi xiaozhengtongluofang can delay the disease process of COPD,and feedback in the clinic,to guide the clinical medication,rich theoretical knowledge of traditional Chinese medicine.Experimental methodsThe experimental animals were selected with SPF grade male wistar rats(1900-210g)85,randomly divided into blank group 10,model group 15,western medicine group 15,Chinese medicine high,medium,low dose group 15.By cigarette smoke joint airway noninvasive intubation drip method to establish model of LPS,smoke for 12 weeks,1 hour each day,each4 in a cage in homemade smoke box to ensure sufficient smoke exposure,2,14,21 weather passage for drip LPS,drip LPS on the same day,don't smoke,don't fill the stomach,don't spray.Smoke first day Chinese traditional medicine group respectively according to high,medium and low dose lavage,model group,the western medicine group with 0.9%NS lavage,western medicine group with budesonide atomization,weigh every week to adjust the dose lavage and atomization time.Blank group without intervention.Experiment the 84th day executed in rats,serum and lung tissue for HE dyeing observation lung organization(airway,lung parenchyma)structure change,structural cells such as airway smooth muscle cells,fibroblasts and epithelial cells,functional cells such as neutrophils,lymphocytes,etc,in the case of a change of Elisa to detect serum IL-6 expression,using immunohistochemical testing means testing JAK1,STAT3,P-the expression of STAT3 protein,reverse transcription polymerase chain reaction(PCR)to detect:JAK1mRNA,STAT3mRNA,SOCS3 gene mRNA expression.Finally,SPASS20.0 was used for statistical analysis to observe the effect of yiqi elimination.3.The results of the study1.Comparison of general conditions of rats.Blank group:rats are larger than other groups obviously,color bright luster,good mental state,active,eating water quantity is big,defecate is normal,the mouth not seen obvious secretions,no respiratory symptoms such as cough,wheezing sound,red tongue;Model group rats:smaller size than other groups,the fur sparse bleak matt,mental state is poor,don't like activity,normally closed,less food into the water,dry stool,or deformed,smoke after the excitement,irritability,can be obviously secretions,oral cough frequency,audible and breathing air entering tone,occasional orbital hemorrhage,tongue color is dark red with blood stasis;Other each intervention group rats:size with the model group,group had low blank,less spiritual,oral cavity is a small amount of secretions,occasional orbital hemorrhage,traditional Chinese medicine(TCM)defecate deformed at high dose group,the other set of defecate normal texture.In the high-dose group,the consumption of drinking water was slightly less than that of other groups,and the other groups had normal drinking water.2.Comparison of lung function results in rats.The drug intervention group all can to delay the lung function decline in different degrees,which in delaying FVC,FEV0.2/FVC,FEV0.2 index the effect of medium dose group of the Chinese medicine is better,the delay PEF indicators,the effect of western medicine groupis the best.3.Comparison of HE staining results in rats.Building has different degree of rat lung tissue inflammatory infiltrates and airway mucosal hyperemia,gas pipe wall,the structure of the alveolar damage,pulmonary bullae formation,smooth muscle cells,goblet cells and fibroblasts hyperplasia,compared with model group,western medicine group and between groups to a lesser extent,Chinese traditional medicine is given to illustrate the effectiveness of the drug intervention,at the same time,the western medicine group in alveoli and airway inflammation and structural damage degree is the lightest,has been clear about the budesonide atomization effect,at the same time,Chinese medicine dosage group in improving the airway inflammation and reduce airway mucosa and alveolar damage effect is good,has great potential for development.4.The expression results of il-6,JAK1,STAT3,JAK1mRNA and STAT3mRNA in rats were compared.The expression of IL-6 of each treatment group were higher than that of blank group,lower than the model group,illustrates the therapy of Budesonide atomization inhalation.And yiqixiaozhengtonglu formula are effective.Chinese medicine middle and high dose group and western medicine group,presenting effective in inhibition of IL-6 is better than the above three groups.At the same time,the difference between Chinese traditional medicine,high dose group and western medicine group was not obvious.In RT-PCR detection,can inhibit JAK1mRNA groups of Chinese medicine and western medicine group,the excessive expression of STAT3mRNA,encourage SOCS3 mRNA expression so as to reduce inflammation,including traditional Chinese medicine(TCM)in the dose group and better effects of western medicine group,difference has statistical significance(P<0.05)4.Research conclusionsMedium dose of yiqixiaozhengtonglu formula can improve cough wheeze,mucus secretion in rats,weight loss,etc.Generally,can inhibit IL-6,JAK1,STAT3,JAK1mRNA STAT3mRNA expression,promote SOCS3 mRNA expression to suppress chronic inflammation,and slow to reduce airway and alveolar damage,delay the process of airway obstruction,a certain extent improve lung function.