Experimental Study On The Effects Of Electroacupuncture On Serum MMP-9 And TIMP-1 In Rabbits With Atherosclerosis

Purpose:To investigate the effect of electroacupuncture at Neiguan,Zusanli and Guanyuan on the expression of MMP-9 and TIMP-1 in serum of rabbits with atherosclerosis(AS)To explore the mechanism of electroacupuncture at Neiguan,Zusanli and Guanyuan acupoints in inhibiting the inflammatory response of atherosclerotic model rabbits.Materials and methods:Twenty-six male New Zealand rabbits were randomly divided into blank group and model group for 1 week.7 rabbits in the blank group and 19 rabbits in the blank group were feed with normal diet.The model group was fed with high fat diet and fed with normal diet every other day.After 4 weeks,the carotid artery balloon injury was performed in the model group.The rabbits were fed with high fat diet for 8 weeks,and then randomly selecte from the blank group and the model group to test whether the model was successful.Methods The histopathological changes of carotid atherosclerotic plaques were observed by HE staining.The model group was randomly divided into model group,acupuncture group and western medicine group.Acupuncture group was treated with internal organs(bilateral),Zusanli(bilateral)and Guan Yuan for 1 day,and the western medicine group was given 1 mg / kg of atorvastatin calcium Day 1,were treated for 4 weeks.The expression of MMP-9 and TIMP-1 in the blood of rabbits was determined by enzyme-linked immunosorbent assay(ELESA).All the common carotid arteries were subjected to HE staining to observe the morphological changes of common carotid arteries in rabbits.Results:1.By observing the pathological sections of each group of rabbits,EA group and western medicine group were less than the model group.2.The level of serum MMP-9 in EA group and western medicine group was significantly lower than that before treatment(P <0.05).3.The level of serum TIMP-1 in EA group and western medicine group was significantly higher than that before treatment(P <0.05).Conclusion:1.Electroacupuncture at the end,Zusanli,Guanyuan acupuncture can improve the common carotid artery atherosclerosis pathological state.2.Electroacupuncture at Neiguan,Zusanli,Guanyuan acupuncture can inhibit the inflammatory response,reduce plaque instability,play the role of anti-atherosclerosis,the mechanism may be related to the regulation of MMP-9 and TIMP-1 imbalance between State related.