Experimental Study On The Effect Of Compound Kushen Injection On Human Breast Cancer Cells MCF-7 And MDA-MB-231

Purpose: Observe compound Kushen injection different state of human breast cancer cells the expression of estrogen receptor MCF-7(ER+)and MDA-MB-231(ER-)proliferation and apoptosis,as compound Kushen injection applied in the experimental basis for clinical treatment of breast cancer.Material and method:In vitro human breast cancer MCF-7(ER +)and MDA-MB-231 cells(ER-),the application of the final concentration of 0.4mg/ml of compound Kushen injection 48 hours respectively deal with two kinds of cells,and set up blank control group,respectively,as well as the application of the final concentration of 2umol/L docetaxel(DXT)deal with the positive control group;Using tetramethyl azo thiazole blue colorimetry(determined by MTT)observation compound Kushen injection on human breast cancer cell MCF-7 and MDA-MB-231 cell proliferation activity of influence;By using in situ end labeling(TUNEL)research compound Kushen injection on human breast cancer cell MCF-7 and MDA-MB-231 the impact of two kinds of cell apoptosis.Results: 1.The compound Kushen injection can obviously inhibit MCF-7 with human breast cancer cells MDA-MB-231 proliferation.2.Compound Kushen injection MCF-7 with human breast cancer cells can be induced apoptosis of MDA-MB-231,compared with the two control group which has significant difference(P < 0.05).Conclusion: 1.The compound Kushen injection in human breast cancer cells in vitro culture MCF-7 and MDA-MB-231 can inhibit proliferation role.2.The compound Kushen injection in human breast cancer cells in vitro culture MCF-7 and MDA-MB-231 has the function of the induction of apoptosis.