In Vitro Study Of The Inhibitory Effect Of Cinnamaldehyde On Pancreatic Cancer Cells

Purpose:To explore Chinese medicine monomer cinnamaldehyde on pancreatic cancer cell PANC-1 proliferation,apoptosis,migration of effect and its molecular mechanism?Material and method:Selection of pancreatic ductal epithelial cancer cells PANC-1the use of vitro cell culture techniques for pancreatic cancer PANC-1 cell in vitro culture,the traditional Chinese medicine monomer cinnamaldehyde reference substance diluted in different concentrations on the PANC-1 cells.By MTT colorimetric method,observation pancreatic cancer cells PANC-1 under the effect of cinnamaldehyde the cell proliferation situation and proliferation conditions change with the change of the concentration and the time of drug.Use the Annexin V-APC / 7 aad apoptosis detection kit by flow cytometry instrument to detect PANC-1 cell apoptosis under the effect of cinnamaldehyde.Observation the migration change of PANC-1 cell under the effect of cinnamaldehyde use the Transwell Chambers model.By Western blot experiment detection of Bcl-2 protein,inactive Caspase-9(Pro Caspase 9)protein and activation of Caspase-9(Cleaved Caspase-9)protein expression which are associated with apoptosis signal path under the effect of cinnamaldehyde.Results:1.Determined by MTT results showed that cinnamaldehyde in pancreatic cancer cells PANC-1 proliferation inhibition(P < 0.05),and with the concentration of the cinnamaldehyde concentration and action time,the Effect is obvious,cinnamaldehyde for 24 hours,48 hours,72 hours is the highest inhibition rate were 62.86%,71.33%,76.47%,half of its inhibition concentration IC50 alue concentration of 11.72,9.43,5.26,respectively.2.Detected by flow cytometry instrument when cinnamic aldehyde cinnamaldehyde concentration with 24 hours IC50 11 ng/ml applies to PANC-1 cell apoptosis can promote PANC-1 apoptosis.cinnamaldehyde group of early apoptosis rate and late apoptosis rate higher than the control group,with statistical significance(P < 0.05).3.Transwell Chambers model observation by cinnamaldehyde can inhibit PANC-1 cell migration ability,different concentration of cinnamaldehyde in cells after 24 hours of drug group compared with control group: the number of PANC-1 cells through the Transwell Chambers decreased significantly,the results have statistical significance(P<0.05),And the results with concentration dependence,the cells which through the small room has changed its form.4.Western blot experiment detected in different concentration cinnamaldehyde function after48 hours,The Bcl-2 protein expression quantity obvious decrease with the increase of drug concentration,and Pro Caspase-9 protein expression quantity obvious decrease with the increase of drug concentration,and Cleaved Caspase-9 protein expression quantity increased significantly with the drug concentration increase.Conclusion:1.Cinnamaldehyde can inhibit pancreatic cancer PANC-1 cell proliferation,inhibition rate according to the concentration time dependence.2.Cinnamaldehyde can promote pancreatic cancer PANC-1 cell apoptosis.3.Cinnamaldehyde can inhibit pancreatic cancer PANC-1 cell the ability of migration.4.Cinnamaldehyde can make apoptosis related proteins the Bcl-2 protein,inactive Caspase-9(Pro Caspase-9)protein expression decreased,activated Caspase-9(Cleaved Caspase-9)protein expression increased.Cinnamaldehyde on pancreatic cancer cell inhibitory effect may be related to apoptosis signaling pathways,the specific molecular mechanism still needs further research.